SCC064
LX-2 Human Hepatic Stellate Cell Line
Human
Synonym(s):
HSC, Perisinusoidal cells, Ito cells, Hepatic lipocytes, Hepatic pericytes
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All Photos(3)
About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.75
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product name
LX-2 Human Hepatic Stellate Cell Line, a highly suitable model of human hepatic fibrosis
biological source
human
Quality Level
technique(s)
cell culture | stem cell: suitable
shipped in
liquid nitrogen
General description
Hepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for human studies.
LX-2 was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passaged cells in low serum media conditions.
Immortalized LX-2 was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.
LX-2 was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passaged cells in low serum media conditions.
Immortalized LX-2 was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.
Application
Research Category
Infectious Diseases
Inflammation & Immunology
Stem Cell Research
Infectious Diseases
Inflammation & Immunology
Stem Cell Research
Components
1) ≥1X106 viable LX-2 Human Hepatic Stellate Cells: (Catalog No. SCC064). Store in liquid nitrogen.
Quality
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negatrive for mycoplasma contamination.
• Cells are tested by PCR and are negative for Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negatrive for mycoplasma contamination.
Storage and Stability
LX-2 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality. LX-2 cells have been successfully expanded past passage 50 in culture.
Other Notes
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the "Academic Use Agreement" as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
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Interleukin-21 mediates hepatitis B virus-associated liver cirrhosis by activating hepatic stellate cells.
Feng, Guohua, et al.
Hepatology Research : the Official Journal of the Japan Society of Hepatology (2013)
Zhiqiang Li et al.
iScience, 24(12), 103449-103449 (2021-12-21)
Glucosylceramide (GluCer) was accumulated in sphingomyelin synthase 1 (SMS1) but not SMS2 deficient mouse tissues. In current study, we studied GluCer accumulation-mediated metabolic consequences. Livers from liver-specific Sms1/global Sms2 double-knockout (dKO) exhibited severe steatosis under a high-fat diet. Moreover, chow
Ching-Fen Wu et al.
Journal of cellular biochemistry, 114(3), 541-550 (2012-09-11)
Hepatitis C virus core protein (HCVcp), which is secreted by infected cells, is reported as an immunomodulator in immune cells. However, the effects of HCVcp on hepatic stellate cells (HSCs), the key cells in liver fibrosis, still remain unclear. In
Ganesh Satyanarayana et al.
Theranostics, 11(19), 9331-9341 (2021-10-15)
Rationale: Fibrosis is a pathologic condition of abnormal accumulation of collagen fibrils. Collagen is a major extracellular matrix (ECM) protein synthesized and secreted by myofibroblasts, composing mainly (Gly-X-Y)n triplet repeats with >30% Gly residue. During fibrosis progression, myofibroblasts must upregulate
TRAIL regulates collagen production through HSF1-dependent Hsp47 expression in activated hepatic stellate cells.
Park, Soo-Jung, et al.
Cellular Signalling, 25, 1635-1643 (2013)
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