MABS193
Anti-v-Src Antibody, clone 327
clone 327, from mouse
Synonym(s):
Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src, pp60c-src, p60-Src
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
327, monoclonal
species reactivity
mouse
technique(s)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SRC(6714)
General description
v-Src is the product of the Rous sarcoma virus (RSV) oncogene, and functions as a non-receptor tyrosine kinase belonging to the family of Src proteins. Srcs are typically activated by dimerized cell surface receptors such as integrins, receptor tyrosine kinases, and cytokine receptors. Activated Src kinases phosphorylate the cytoplasmic domains of these receptors enabling interaction between these receptors and their downstream effector molecules. Src proteins are involved in many cellular processs such as apoptosis, immunity, gene expression, cell adhesion, cell migration, and cell transformation. Previous studies have reported that v-Src plays an important role in tumorigenesis.
Immunogen
Recombinant protein corresponding to purified pp60src from bacterial recombinants which direct the synthesis of v-Src.
Application
Anti-v-Src Antibody, clone 327 detects level of v-Src & has been published & validated for use in Western Blotting.
Research Category
Signaling
Signaling
Research Sub Category
Immunological Signaling
Immunological Signaling
Quality
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected v-Src in 10 µg of NIH/3T3 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected v-Src in 10 µg of NIH/3T3 cell lysate.
Target description
~60 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
NIH/3T3 cell lysate
NIH/3T3 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Lilian Varricchio et al.
Molecular cancer research : MCR, 5(11), 1213-1221 (2007-11-21)
This report offers direct evidence that association of the estradiol receptor (ER) with Src triggered by steroid agonists or growth factors controls breast and prostate cancer cell growth. This association is abolished in whole cells and in vitro by a
Gabriella Castoria et al.
PloS one, 8(10), e76899-e76899 (2013-10-17)
Hormones and growth factors influence the proliferation and invasiveness of human mesenchymal tumors. The highly aggressive human fibrosarcoma HT1080 cell line harbors classical androgen receptor (AR) that responds to androgens triggering cell migration in the absence of significant mitogenesis. As
Eynat Finkelshtein et al.
Molecular biology of the cell, 25(11), 1808-1818 (2014-04-04)
Female mice lacking protein tyrosine phosphatase ε (PTP ε) are mildly osteopetrotic. Osteoclasts from these mice resorb bone matrix poorly, and the structure, stability, and cellular organization of their podosomal adhesion structures are abnormal. Here we compare the role of
June X Zou et al.
The Journal of biological chemistry, 277(3), 1824-1827 (2001-10-30)
One of the prominent effects of the Src kinase is to reduce cell adhesion. The small GTPase, R-Ras, affects cell adhesion by maintaining integrin activity, and the ability of R-Ras to do so can be regulated by phosphorylation of a
Hsp90 inhibition transiently activates Src kinase and promotes Src-dependent Akt and Erk activation.
Fumitaka Koga et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(30), 11318-11322 (2006-07-18)
Hsp90 plays an essential role in maintaining stability and activity of its clients, including oncogenic signaling proteins that regulate key signal transduction nodes. Hsp90 inhibitors interfere with diverse signaling pathways by destabilizing and attenuating activity of such proteins, and thus
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