MABE1115M

Anti-Pax1 Antibody, clone 5A2

clone 5A2, from rat

• Synonym(s): Paired box protein Pax-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5A2, monoclonal
• species reactivity: human, mouse
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_032806
• UniProt accession no.: P09084
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: mouse ... Pax1(18503)

Description

General description

Paired box protein Pax-1 (UniProt: P09084) is encoded by the Pax1 (also known as Pax-1) gene (Gene ID: 18503) in murine species. Pax-1 is a transcriptional activator that plays a role in the formation of segmented structures of the embryo and has important role in the normal development of the vertebral column. Mutations in Pax1 gene are associated with vertebral malformations. Undulated homozygous mice exhibits vertebral malformations along the entire rostro-caudal axis. Pax genes are vital for the specification of progenitor cells and for maintenance of progenitor cell fate, including that of cancer cells. Pax1 gene is silenced by methylation in ovarian and cervical cancers and may serve as a tumor suppressor gene. The methylation levels of Pax1 gene is significantly higher in tumor tissues compared to non-tumor paracancerous tissues. Hence, methylation status of Pax1 may serve as useful biomarkers for the detection of cervical cancer and is also a good prognostic indicator for oral squamous cell carcinoma. (Ref.: Huang J et al (2017). Int. J. Environ. Res. Public Health. 14(2). pii: E216).

Specificity

Clone 5A2 detects Paired box protein Pax-1 in murine tissues.

Immunogen

GST-tagged full-length murine recombinant paired box protein Pax-1.

Application

Anti-Pax1, clone 5A2, Cat. No. MABE1115, is a highly specific rat monoclonal antibody that targets Paired box protein Pax-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Western Blotting Analysis: A representative lot detected Pax1 in Western Blotting applications (Feederle, R., et. al. (2016). Monoclon Antib Immunodiagn Immunother. [Epub ahead of print]).

Immunohistochemistry Analysis: A representative lot detected Pax1 in Immunohistochemistry applications (Feederle, R., et. al. (2016). Monoclon Antib Immunodiagn Immunother. [Epub ahead of print]; Kist, R., et. al. (2014). PLoS Genet. 10(10):e1004709).

Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Pax1 in Embryonic mouse head (E14.5) (Courtesy of Dr. Heiko Peters, Ph.D., Institute of Genetic Medicine, Newcastle University, United Kingdom).

Western Blotting Analysis: A 1:10 dilution from a representative lot detected Pax1 in d11.5 embryonic vertebral column tissue lysates (Courtesy of Dr. Heiko Peters, Ph.D., Institute of Genetic Medicine, Newcastle University, United Kingdom).

Quality

Evaluated by Western Blotting in mouse esophagus tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Pax1 in 10 µg of mouse esophagus tissue lysate.

Target description

~46 kDa observed; 46.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable