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53702

Millipore

Pronase® Protease, Streptomyces griseus

Synonym(s):

Pronase® powder

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54
Pricing and availability is not currently available.

form

lyophilized

Quality Level

specific activity

≥45,000 proteolytic units/g dry wt

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)

solubility

water: 10 mg/mL

shipped in

ambient

storage temp.

2-8°C

General description

A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.
A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but stable over wide range of pH and temperature. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Physical form

Lyophilized from a solution containing calcium acetate as a stabilizer.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.

Other Notes

Narahashi, Y., et al. 1968. J. Biochem. 64, 427.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The preparation and characterization of cell walls and the preparation of flagella of Vibrio fetus.
R F Keeler et al.
Journal of general microbiology, 43(3), 439-454 (1966-06-01)
T E Davis et al.
Infection and immunity, 15(3), 978-987 (1977-03-01)
Enzymes capable of hydrolyzing cell walls of Blastomyces dermatitidis and chemotypes I and II of Histoplasma capsulatum were prepared in the laboratory or obtained from commercial sources. They included chitinases, beta-1,3-glucanases, beta-1,6-glucanase, and Pronase. Monosaccharides and disaccharides of glucose released
Nicole M Templeman et al.
Developmental cell, 54(1), 92-105 (2020-06-17)
Evolutionarily conserved signaling pathways are crucial for adjusting growth, reproduction, and cell maintenance in response to altered environmental conditions or energy balance. However, we have an incomplete understanding of the signaling networks and mechanistic changes that coordinate physiological changes across
Aimee E Stephenson et al.
Molecular microbiology, 43(1), 147-157 (2002-02-19)
Streptococcus parasanguis is a primary colonizer of the tooth surface and plays a pivotal role in the formation of dental plaque. The fimbriae of S. parasanguis are important in mediating adhesion to saliva-coated hydroxylapatite (SHA), an in vitro tooth adhesion
Kenya Terabe et al.
The Journal of biological chemistry, 294(47), 17799-17817 (2019-10-18)
Hyaluronan is a critical component of articular cartilage and partially helps retain aggrecan within the extracellular matrix of this tissue. During osteoarthritis, hyaluronan and aggrecan loss are an early sign of tissue damage. However, our recent attempts to mimic hyaluronan

Questions

  1. What is the difference between these two products of yours? first: SKU no. 10165921001 second SKU no. 53702-10KU

    1 answer
    1. The manufacturer/tradename of PRON-RO (10165921001) is Roche where as the manufacturer/tradename of 53702 is Calbiochem®. The SKU and pack sizes are also different for both.
      Answer Source:
      1.https://www.sigmaaldrich.com/IN/en/product/roche/pron-ro
      2. https://www.sigmaaldrich.com/IN/en/product/mm/53702

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