203895

BTS

A potent inhibitor of Ca²⁺-stimulated myosin S1 ATPase (IC₅₀ = ~ 5 µM) and reversibly blocks the gliding motility.

• Synonym(s): BTS, N-Benzyl- p-toluenesulphonamide, N-Benzyl-p-toluenesulphonamide
• Empirical Formula (Hill Notation): C₁₄H₁₅NO₂S
• CAS Number: 1576-37-0
• Molecular Weight: 261.34
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: crystalline solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: white
• solubility: DMSO: 200 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C14H15NO2S/c1-12-7-9-14(10-8-12)18(16,17)15-11-13-5-3-2-4-6-13/h2-10,15H,11H2,1H3
• InChI key: WTHKAJZQYNKTCJ-UHFFFAOYSA-N

Description

General description

A potent inhibitor of Ca²⁺-stimulated myosin S1 ATPase (IC₅₀ = ~ 5 µM) and reversibly blocks the gliding motility. It also weakens myosin’s interaction with F-actin. Much less effective in suppressing contraction in rat myocardial or rabbit slow twitch muscle and has no effect on platelet myosin II.

An aryl sulphonamide that acts as a potent inhibitor of Ca²⁺-stimulated myosin S1 actin-stimulated ATPase activity (IC₅₀ = ~5 µM). Also blocks actin-stimulated ATPase activity with similar potency (IC₅₀ = 5 µM). Reversibly blocks gliding motility of skeletal muscle myosin (IC₅₀<2 µM). Less effective in suppressing contraction in rat myocardial and rabbit slow twitch muscle compared to skeletal muscle. Does not inhibit platelet myosin II.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Ca²⁺-stimulated myosin S1 ATPase

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: ~5 µM against Ca2+-stimulated myosin S1 ATPase

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Cheung, A., et al. 2002. Nat. Cell Biol.4, 83.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable