For the selective enrichment of samples for Salmonella sp. in accordance to EP
form
liquid (ready-to-use)
feature
closure type screw cap with septum ready-to-use
composition
with soy peptone
packaging
single packed of 20 or 100 tubes per box tube of
technique(s)
direct inoculation: suitable sterile filtration: suitable
capacity
10 mL
tube capacity
17 mL
tube filling volume
10 mL
pH
5.2
application(s)
microbiology pharmaceutical
storage temp.
2-25°C
suitability
Salmonella spp.
General description
Rappaport Vassiliadis medium is used for the selective enrichment and presumptive detection of Salmonella sp. This medium is a modification of Rappaport-Vassiliadis enrichment broth and is used for motility enrichment in Petri dishes. It is a rapid and sensitive medium for the isolation of Salmonella sp. and can be seeded directly after pre-enrichment.
Application
Rappaport Vassiliadis medium is used for the selective enrichment and presumptive detection of Salmonella sp. in pharmaceutical products.
Analysis Note
Soy Peptone provides nitrogenous, carbonaceous, B complex vitamins and other essential growth nutrients. Phosphate provides buffering capacity to the medium. Malachite green serves as a selective agent. Sodium chloride maintains the osmotic equilibrium of the media. Salmonella generally survives a slightly high osmotic pressure (due to MgCl2 in the medium), grows at a slightly low pH, and is resistant to malachite green compared to other bacteria, thus making it a selective media for the growth of Salmonella sp.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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