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Sigma-Aldrich

Bovine Serum Albumin

10% Aqueous Solution, Nuclease-Free

Synonym(s):

Albumin, Bovine Serum, 10% Aqueous Solution, Nuclease-Free

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About This Item

CAS Number:
9048-46-8
UNSPSC Code:
12352202
NACRES:
NA.25

product name

Albumin, Bovine Serum, 10% Aqueous Solution, Nuclease-Free,

form

liquid

Quality Level

300

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤0.02% Fatty acids
≤10 ppm Heavy metal
≤2.0% Ash

foreign activity

Nuclease, none detected
Protease, none detected

shipped in

ambient

storage temp.

2-8°C

General description

Purified chromatographically to reduce DNases and RNases to exceptionally low levels.
Useful for applications in which acetylated BSA is not desirable, such as in antibody dilution, DNA footprinting and gel shift assay, ELISA, enzyme assay, enzyme stabilization, immunoblotting, immunofluorescence, PCR, restriction enzyme reactions, RIA, probe-based diagnostics, radioactive quenching, and receptor binding studies. Purified chromatographically to reduce DNases and RNases to exceptionally low levels.
Useful for applications in which acetylated BSA is not desirable, such as in antibody dilution, DNA footprinting, and gel shift assay, ELISA, enzyme assay, enzyme stabilization, immunoblotting, immunofluorescence, PCR, restriction enzyme reactions, RIA, probe-based diagnostics, radioactive quenching, and receptor binding studies.

Warning

Toxicity: Standard Handling (A)

Physical form

10% aqueous solution, 0.22 µm-filtered, pH 6.8-7.2.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Alan J Simmons et al.
STAR protocols, 3(3), 101570-101570 (2022-07-27)
In droplet-based single-cell RNA-sequencing (scRNA-seq) experiments, cells, along with some of their surrounding buffer and ambient material, are encapsulated into droplets for mRNA capture and barcoding. This protocol details the steps for human gut tissue dissociation using cold active protease
Timothy T Harden et al.
Nature communications, 11(1), 448-448 (2020-01-25)
RNA polymerases (RNAPs) transcribe genes through a cycle of recruitment to promoter DNA, initiation, elongation, and termination. After termination, RNAP is thought to initiate the next round of transcription by detaching from DNA and rebinding a new promoter. Here we
Larry J Friedman et al.
Biophysical journal, 91(3), 1023-1031 (2006-05-16)
Complexes of macromolecules that transiently self-assemble, perform a particular function, and then dissociate are a recurring theme in biology. Such systems often have a large number of possible assembly/disassembly intermediates and complex, highly branched reaction pathways. Measuring the single-step kinetic
Larry J Friedman et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(24), 9740-9745 (2013-05-31)
Sequence-specific DNA binding proteins must quickly bind target sequences, despite the enormously larger amount of nontarget DNA present in cells. RNA polymerases (or associated general transcription factors) are hypothesized to reach promoter sequences by facilitated diffusion (FD). In FD, a
Larry E Tetone et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(7), E1081-E1090 (2017-02-01)
The secondary channel (SC) of multisubunit RNA polymerases (RNAPs) allows access to the active site and is a nexus for the regulation of transcription. Multiple regulatory proteins bind in the SC and reprogram the catalytic activity of RNAP, but the
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