1.04194

Fast green FCF (C.I. 42053)

• Empirical Formula (Hill Notation): C₃₇H₃₄N₂Na₂O₁₀S₃
• CAS Number: 2353-45-9
• Molecular Weight: 808.85
• MDL number: MFCD00013053
• NACRES: NA.21

Properties

• form: solid
• Quality Level: 200
• potency: >2000 mg/kg LD₅₀, oral (Rat)
• composition: Dye, ≥85% spectrophotometric assay
• pH: 2.7 (20 °C, 10 g/L in H₂O)
• mp: 290 °C (decomposition)
• solubility: 150 g/L
• bulk density: 350 kg/m³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3-,40,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
• InChI key: XJBPDZVCYTYRIN-UHFFFAOYSA-L

Description

General description

The dry dye Fast Green FCF (C.I. 42053) for microscopy Certistain^®, is used for the preparation of a staining solution that can be used for modified connective tissue staining acc. to van Gieson in histological sections. Further it is applied in histology as counterstaining in e.g. trichrome staining acc. to Masson and additionally for histochemical staining, as well as in Cytology as counterstain instead of light green SF in Papanicolaou′s staining. As CERTISTAIN^® dye it is chemically quality-certified according to highest standards and strict specifications.

Application

for microscopy Certistain^®

Analysis Note

Dye content (spectrophotometrically): ≥ 85 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 622 - 626 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 1360 - 1610
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Warning
• Hazard Statements: H335 - H341
• Precautionary Statements: P202 - P261 - P271 - P280 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Muta. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): 352.4 °F - closed cup
• Flash Point(C): 178 °C - closed cup