The enrichment medium must be purchased separately: 1041430001 Bolton Broth and 1041430001 Bolton Broth Selective Supplement.
Sample Material / Sample Enrichment
• Add 25g solid sample to 225ml Bolton enrichment broth in a 250ml polystyrene bottle or 25ml liquid sample to 200ml Bolton enrichment broth in a 250ml polystyrene bottle.
• If necessary, transfer to the filter unit of a stomacher bag and homogenize in stomacher for l minute.
• Transfer homogenate back to 250ml polystyrene bottle, ensuring a headspace of 10-15 % is provided (see Note below).
• Discard the stomacher bag and filter unit.
• Incubate for 4hr at 37 degC.
• Transfer to 41.5 degC and incubate for a further 44hr.
Note: If headspace is more than 15%, incubate enrichment culture microaerophilically, using a controlled atmosphere chamber. Anaerocult C gas packs (1.32383.0001 or 1.32370.0001) can be used to generate this.