07-716-I
Anti-KDR/Flk-1/VEGFR2 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, FLK-1, Kinase NYK, Protein-tyrosine kinase receptor flk-1, CD309
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
clone:
polyclonal
application:
WB
species reactivity:
mouse, human
technique(s):
western blot: suitable
citations:
2
Pricing and availability is not currently available.
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
technique(s)
western blot: suitable
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KDR(3791)
mouse ... Kdr(16542)
General description
The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.
Immunogen
Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of mouse KDR/Flk-1/VEGFR2.
Application
Detect KDR/Flk-1/VEGFR2 using this rabbit polyclonal antibody, Anti-KDR/Flk-1/VEGFR2 Antibody validated for use in western blotting.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
Western Blotting Analysis: A 1:500 dilution from a representative lot detected KDR/Flk-1/VEGFR2 in 10 µg of PAE cells transfected with CSF/VEGFR2 chimera.
Quality
Evaluated by Western Blotting in HUVEC cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected KDR/Flk-1/VEGFR2 in 10 µg of HUVEC cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected KDR/Flk-1/VEGFR2 in 10 µg of HUVEC cell lysate.
Target description
~230 kDa observed
Linkage
Replaces: 07-716
Physical form
Affinity purified
Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Chike Osude et al.
Cells, 11(10) (2022-05-29)
NSCLC treatment includes targeting of EGFR with tyrosine kinase inhibitors (TKIs) such as Erlotinib; however, resistance to TKIs is commonly acquired through T790M EGFR mutations or overexpression of vascular endothelial growth factor receptor-2 (VEGFR-2). We investigated the mechanisms of EGFR-TKI
Edward Hartsough et al.
iScience, 25(8), 104736-104736 (2022-08-10)
Through tightly controlled multilayer mechanisms, vascular endothelial growth factor receptor-2 (VEGFR-2) activation and its downstream signal transduction govern vasculogenesis and pathological angiogenesis, such as tumor angiogenesis. Therefore, it is critical to understand the molecular mechanisms governing VEGFR-2 signal transduction. We
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