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Key Documents

07-102

Sigma-Aldrich

Anti-IGF2BP1/2 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Hepatocellular carcinoma autoantigen p62, IGF-II mRNA-binding protein 2, IGF2 mRNA-binding protein 2, VICKZ family member 2, insulin-like growth factor 2 mRNA binding protein 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rat (based on 100% sequence homology)

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

General description

Insulin-like growth factor 2 mRNA-binding proteins are RNA-binding factors belonging to the IMP (IGF-II mRNA-binding protein) family of proteins. These proteins contain four K homology domains and two RNA recognition motifs. IGF2BP′s have key roles in regulating the translation of target mRNAs. For the insulin-like growth factor 2 (IGF2) gene, IGF2BP′s bind to the 5′ UTR of the mRNA and regulate translation. IGF2BP′s are expressed in a variety of fetal tissues such as; follicles in ovary, liver, lung, kidney, thymus, placenta. Expression has also been observed in the gonoocytes of testis, oocytes, semen, spermatagonia and in several cancers including; testicular, lung, kidney, trachea, and prostate.

Specificity

This antibody recognizes IGF2BP1 and IGFBP2 at the C-terminus.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to human IGF2BP1/2 at the C-terminus.

Application

Anti-IGF2BP1/2 Antibody is a Rabbit Polyclonal Antibody for detection of IGF2BP1/2 also known as Hepatocellular carcinoma autoantigen p62, IGF2 mRNA-binding protein 2 & has been validated in WB & IP.
Research Category
Apoptosis & Cancer
Research Sub Category
RNA Metabolism & Binding Proteins

RNA Metabolism & Binding Proteins
Western Blot (SNAP ID) Analysis: 4 µg/mL from a representative lot detected IGF2BP1/2 on 10 µg of HEK293 cell lysate.

Immunoprecipitation Analysis: 4 µg/mL from a representative lot was used by an independent laboratory in IP.

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected IGF2BP1/2 on 10 µg of HEK293 cell lysate.

Target description

~63 kDa observed. Reactivity has been observed for both IGF2BP1 and IGF2BP2

Physical form

Affinity purified
Purified rabbit polyclonal containing PBS with 0.09% azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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