206342

Celestine blue

Dye content 80 %

• Synonym(s): Mordant Blue 14
• Empirical Formula (Hill Notation): C₁₇H₁₈ClN₃O₄
• CAS Number: 1562-90-9
• Molecular Weight: 363.80
• Colour Index Number: 51050
• Beilstein: 3830911
• EC Number: 216-346-2
• MDL number: MFCD00011927
• UNSPSC Code: 12171500
• PubChem Substance ID: 24852395
• NACRES: NA.47

Properties

• form: powder
• composition: Dye content, 80%
• mp: 227-230 °C
• solubility: water: 0.1%
• λmax: 642 nm
• ε (extinction coefficient): ≥29000 at 640-650 nm in water at 0.01 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)c(O)c(O)cc3C(N)=O
• InChI: 1S/C17H17N3O4.ClH/c1-3-20(4-2)9-5-6-11-13(7-9)24-16-14(19-11)10(17(18)23)8-12(21)15(16)22;/h5-8H,3-4H2,1-2H3,(H3-,18,19,21,22,23);1H
• InChI key: VXAFJUDTZSMEFN-UHFFFAOYSA-N

Description

Application

May be substituted for hematoxylin in hematoxylin and eosin procedures.

Biochem/physiol Actions

Celestine blue (CB) is an oxazine dye and is used in combination with the alum hematoxylin, as a nuclear stain. It gives good nuclear definition and is resistant to strong acid dyes. Celestine blue is commonly used for routine staining of fixed sections. It has been used as a new electroactive indicator in DNA biosensors.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves