T3411
Tetramethylammonium chloride solution
for molecular biology
Synonym(s):
N,N,N-Trimethylmethanaminium chloride
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352107
PubChem Substance ID:
NACRES:
NA.31
grade
for molecular biology
Quality Level
concentration
5 M
foreign activity
DNase, RNase, none detected
SMILES string
[Cl-].C[N+](C)(C)C
InChI
1S/C4H12N.ClH/c1-5(2,3)4;/h1-4H3;1H/q+1;/p-1
InChI key
OKIZCWYLBDKLSU-UHFFFAOYSA-M
General description
Tetramethylammonium binds AT-rich DNA polymers while concomitantly abolishing the preferential melting of AT versus GC base pairs. It is supplied as a 0.2 μm filtered solution in 18 megohm water.
Application
Tetramethylammonium chloride solution (TMAC) has been used:
- in the preparation of hybridization cocktail for array hybridization and scanning
- in next-generation sequencing (NGS), and genome-wide unbiased identification of double-stranded breaks enabled by sequencing (GUIDE-seq) library preparation
- in the preparation of TMAC buffer and bead hybridization mixture for hybridization and detection
Certificates of Analysis (COA)
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