SAB4200465

Anti-Kindlin-1 antibody, Mouse monoclonal

clone KN-4, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-C20orf42, Monoclonal Anti-DTGCU2, Monoclonal Anti-FERMT1 URP1, Monoclonal Anti-KIND1, Monoclonal Anti-Kindlin-1 antibody produced in mouse, Monoclonal Anti-UNC112A, Monoclonal Anti-fermitin family member 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: KN-4, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa (isoform 4); antigen ~60 kDa (isoform 2); antigen ~77 kDa (isoform 1)
• species reactivity: mouse, human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 2.5-5.0 μg/mL using SW-620 cells; western blot: 1.0-2.0 μg/mL using SW-48 total cell extracts
• isotype: IgG1
• UniProt accession no.: Q9BQL6
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FERMT1(55612) ; mouse ... Fermt1(241639)

Description

General description

Kindlin-1 is coded by FERMT1 (fermitin family member 1) gene. It is a focal adhesion protein, expressed in the skin and intestine. FERMT1 is located on human chromosome 20p.12.3.

Immunogen

synthetic peptide corresponding to a sequence close to the N-terminus of human Kindlin-1, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Kindlin-1 antibody has been used in immunohistochemistry, immunofluorescence staining and western blotting.

Biochem/physiol Actions

Kindlin-1 protein, coded by FERMT1 gene modulates keratinocyte electrotaxis. It participates in the initiation of the integrin family of extracellular matrix receptors. FERMT1 maintains the lamellipodial protrusions at the time of electrotaxis. Mutations in kindlin-1 results in Kindler syndrome (KS).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable