S0191

Sonic Hedgehog Peptide from mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, Fused to a histidine tag at the C-terminus, lyophilized powder

• Synonym(s): Shh
• MDL number: MFCD03792594
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.4-3.0 μg/mL ED₅₀
• mol wt: predicted mol wt 20 kDa
• packaging: pkg of 25 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: <1 EU/μg
• UniProt accession no.: Q62226
• storage temp.: −20°C
• Gene Information: mouse ... Shh(20423)

Description

General description

Sonic hedgehog (Shh) is a morphogenic glycoprotein. The gene encoding it is localized on mouse chromosome 5.

Application

Sonic hedgehog peptide from mouse has been used for neural differentiation.

Biochem/physiol Actions

Cell signaling peptide involved in embryonic morphogenic development of several tissues including Hensen′s node, the notochord, and the neural tube.

Sonic hedgehog (Shh) has a role in fracture healing and regeneration. The protein associates with its receptor Patched (Ptc-1), de-represses Smoothened (Smo) and activates the Gli family of transcription factors. Mutations in the Shh pathway have been linked to developmental diseases and cancer.

Other Notes

N-terminal peptide encoding amino acid residues 25-198.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline, pH 7.4, containing 5% trehalose and 50 μg of bovine serum albumin per 1 μg of cytokine.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable