P9577

PCR 50 - 2,000 bp Marker

for electrophoresis of PCR fragments

• Synonym(s): 50-2000 bp ladder, PCR ladder for agarose gel electrophoresis, PCR marker for agarose gel electrophoresis, RTU PCR DNA ladder, RTU PCR DNA marker, ready to use DNA ladder, ready to use DNA marker
• UNSPSC Code: 41105337
• NACRES: NA.25

Properties

• Quality Level: 200
• form: liquid
• usage: sufficient for 50 uses
• suitability: suitable for electrophoresis (DNA)
• storage temp.: −20°C

Description

General description

Sigma′s PCR 50 - 2,000 bp Marker contains 8 dsDNA fragments from 50 – 2,000 bases, evenly spaced. Five μl of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

PCR 50 - 2,000 bp Marker has been used as a marker for DNA gel electrophoresis.

Components

Sigma′s PCR 50 - 2,000 bp Marker is provided as two tubes:

• 1 vial (250 μl volume) PCR Marker
• 1 vial 6× loading buffer (250 μl volume) for diluting samples

Other Notes

Contains 8 bands, 50–2,000 bp in predictably spaced (ladder) double-stranded DNA fragments.

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves