93773

TWEEN^® 20

viscosity 250-450 mPa.s (25 °C)

• Synonym(s): Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
• CAS Number: 9005-64-5
• MDL number: MFCD00165986
• UNSPSC Code: 12161900
• NACRES: NA.21

Properties

• description: non-ionic
• form: viscous liquid
• mol wt: ~1228
• technique(s): DNA extraction: suitable; RNA extraction: suitable; cell culture | mammalian: suitable; protein purification: suitable
• refractive index: n20/D 1.468 (lit.); n20/D 1.471
• viscosity: 250-450 mPa.s(25 °C)
• HLB value: 16.7±1.0
• density: 1.11 g/mL at 20 °C; 1.095 g/mL at 25 °C (lit.)
• SMILES string: CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
• InChI: 1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
• InChI key: HMFKFHLTUCJZJO-UHFFFAOYSA-N

Description

General description

Tween^®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

Tween^® 20 is widely used in several biochemical applications. It has been used:

• as an emulsifying agent for the preparation of stable oil-in-water emulsions
• in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• as a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1%
• for lysing mammalian cells at a concentration of 0.005 to 0.5%
• for rehydrating tissue before in situ hybridization

Non-ionic detergent

Biochem/physiol Actions

Tween^® 20, functioning as a detergent, potentially operates by binding to the hydrophobic components of water-insoluble substances, thereby making them hydrophilic.

Features and Benefits

• Suitable for protein purification and DNA and RNA extraction
• Suitable for mammalian cell culture
• High purity product for research applications

Caution

may get turbid on storage, heat to clear

Other Notes

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Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 527.0 °F - Pensky-Martens closed cup
• Flash Point(C): 275 °C - Pensky-Martens closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves