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MABS2291

Sigma-Aldrich

Anti-DPP4/CD26 Antibody, clone KU44.13A

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

KU44.13A, monoclonal

mol wt

calculated mol wt 88.28 kDa

purified by

using protein G

species reactivity

human

packaging

antibody small pack of 100 μL

technique(s)

ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

isotype

IgG2κ

epitope sequence

Extracellular domain

Protein ID accession no.

UniProt accession no.

shipped in

2-8°C

target post-translational modification

unmodified

General description

Dipeptidyl peptidase 4 (UniProt: P27487; also known as EC:3.4.14.5, ADABP, Adenosine deaminase complexing protein 2, ADCP-2, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26, TP103, CD26) is encoded by the DPP4 (also known as CD26, ADCP2) gene (Gene ID: 1803) in human. DPP4 is a single-pass type II membrane glycoprotein that is ubiquitously expressed on the surface of a variety of cells. It is expressed in endothelial cells, fibroblasts, and lymphocytes and on the apical surfaces of epithelial and acinar cells. It serves as a serine exopeptidase that cleaves X-proline or X-alanine dipeptides from the N-terminus of polypeptides. A soluble form of DPP4 is also present (aa 39-766) that is derived from the membrane by proteolytic processing. DPP4 expression is substantially dysregulated in a variety of disease states including inflammation, cancer, obesity, and diabetes and its inhibition by the gliptin family of drugs has gained importance for the therapy of type 2 diabetes. DPP4 is also involved in the costimulatory signal that is essential for T-cell receptor (TCR)-mediated T-cell activation. Its levels are up-regulated by IL-12 on activated T-cells and down-regulated by TNF. DPP4 also plays a role in tumor development and its effect depends on the tumor type and its microenvironment. It has a tumor suppressor effect in several cancers including melanoma, ovarian cancer, non-small cell lung cancer, prostate cancer, endometrial cancer, and neuroblastoma. Higher levels of DPP4 have been observed in pancreatic cancer and knockdown of DPP4 expression inhibits cell growth, migration, and invasion. (Ref.: Arias-Pinilla, GA., et al. (2020). Sci. Rep. 10; 537; Röhrborn, D., et al. (2015). Front. Immunol. 6; 386).

Specificity

Clone KU44.13A is a mouse monoclonal antibody that detects Dipeptidyl peptidase 4 (DPP4)/CD26.

Immunogen

CFPAC-1 human pancreatic cancer cells and SP2 myeloma cells.

Application

Quality Control Testing

Evaluated by Flow Cytometry in HPAF-II cells.

Flow Cytometry Analysis: 1 μg of this antibody detected DPP4/CD26 in one million HPAF-II cells.

Tested Applications

Immunofluorescence Analysis: A representative lot detected DPP4/CD26 in Immunofluorescence applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

Immunoprecipitation Analysis: A representative lot immunoprecipitated DPP4/CD26 in Immunoprecipitation applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep.10(1):537).

ELISA Analysis: A representative lot detected DPP4/CD26 in ELISA applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

Flow Cytometry Analysis: A representative lot detected DPP4/CD26 in Flow Cytometry applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

Immunohistochemistry Applications: A representative lot detected DPP4/CD26 in Immunohistochemistry applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
This Anti-DPP4/CD26, clone KU44.13A, Cat. No. MABS2291, is a mouse monoclonal antibody that detects DPP4/CD26 and is tested for use in ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Immunoprecipitation.

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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