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CC065

Sigma-Aldrich

Human Tenascin

from human glioma cell line (U251), liquid, 0.1 mg/mL, suitable for cell culture

Synonym(s):

TN-C, Tenascin, Hexabrachion, Cytotactin, Neuronectin

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

product name

Human Tenascin-C Purified Protein,

biological source

human

Quality Level

100
300

Assay

>97% (SDS-PAGE)

form

liquid

mol wt

280—300 kDa

manufacturer/tradename

Chemicon®

concentration

0.1 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)

solubility

water: soluble

NCBI accession no.

UniProt accession no.

Binding Specificity

Peptide Source: Fibrinogen

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... TNC(3371)

General description

Product Source: Human glioma cell line (U251).
Purified Human Tenascin-C Protien. Tenascin is known to: 1) play an active role in development of central nervous system and mesenchymal-derived organs; 2) exist in vasculature of tumors in adults; and 3) function in cell adhesion. By SDS-PAGE analysis, the protein migrates around 280-300kD.

Application

Cell adhesion assays.

Optimal working dilutions must be determined by end user.

Linkage

Replaces: CC066

Physical form

Purified protein supplied as a liquid in PBS containing no preservatives.

Storage and Stability

Maintain at -70ºC for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Ting-Yu Huang et al.
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Oncoimmunology, 7(10), e1478647-e1478647 (2018-10-06)
The dismal prognosis of glioblastoma is attributed in part to the existence of stem-like brain tumor-initiating cells (BTICs) that are highly radio- and chemo-resistant. New approaches such as therapies that reprogram compromised immune cells against BTICs are needed. Effective immunotherapies
Cecelia C Yates et al.
Cell transplantation, 26(1), 103-113 (2016-07-28)
Mesenchymal stem cells (MSCs) remain of great interest in regenerative medicine because of their ability to home to sites of injury, differentiate into a variety of relevant lineages, and modulate inflammation and angiogenesis through paracrine activity. Many studies have found
Mainak Das et al.
Biomaterials, 31(18), 4880-4888 (2010-03-30)
Neuromuscular junction (NMJ) formation, occurring between motoneurons and skeletal muscle, is a complex multistep process involving a variety of signaling molecules and pathways. In vitro motoneuron-muscle co-cultures are powerful tools to study the role of different growth factors, hormones and
Melanie Rodrigues et al.
Tissue engineering. Part A, 19(17-18), 1972-1983 (2013-04-02)
Multipotential stromal cells/mesenchymal stem cells (MSCs) are attractive candidates for regenerative therapy due to the ability of these cells to differentiate and positively influence neighboring cells. However, on implantation for wound reconstruction, these cells are lost as they are challenged

Protocols

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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