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Astec® CHIROBIOTIC® T Chiral (5 μm) HPLC Columns

L × I.D. 10 cm × 4.6 mm, HPLC Column

Synonym(s):

CHIROBIOTIC T Chiral Chromatography Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm

material

stainless steel column

Quality Level

Agency

suitable for USP L63

description

HPLC column

product line

Astec®

packaging

pkg of 1 ea

manufacturer/tradename

Astec®

parameter

0-45 °C temperature
241 bar pressure (3500 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

10 cm × 4.6 mm

matrix

High-purity silica gel particle platform
fully porous particle

matrix active group

teicoplanin glycopeptide phase

particle size

5 μm

pore size

100 Å

operating pH range

3.8-6.8

separation technique

chiral

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General description

CHIROBIOTIC® T and T2 have teicoplanin as the chiral selector. They offer unique selectivity for a number of classes of molecules, specifically underivatized α, β, γ and cyclic amino acids, N-derivatized amino acids, hydroxy-carboxylic acids, acidic compounds including carboxylic acids and phenols, small peptides, neutral aromatic analytes and cyclic aromatic and aliphatic amines. Separations normally obtained on a chiral crown ether or ligand exchange-type CSPs are also possible on the CHIROBIOTIC® T and T2, but in much simpler mobile phases, like water-alcohol. In addition, all of the known beta-blockers (amino alcohols), and dihydrocoumarins have been resolved. CHIROBIOTIC® T and T2 differ in their bonding chemistry and the pore size of the support particle, giving them different selectivity and preparative capacity.

  • Bonded phase: Teicoplanin
  • Operating pH range: 3.8 - 6.8
  • Particle diameter: 5, 10 or 16 μm
  • Pore size: 100 Å (CHIROBIOTIC® T) or 200 Å (CHIROBIOTIC® T2)

CHIROBIOTIC FAQs
CHIROBIOTIC Reference Bibliography
Chiral Product Literature

Application


  • Chiral liquid chromatography-mass spectrometry (LC-MS/MS) method development for the detection of salbutamol in urine samples.: This study utilizes the Astec CHIROBIOTIC T Chiral HPLC Column to develop an LC-MS/MS method for detecting and quantifying salbutamol enantiomers in urine samples. This application demonstrates the column′s utility in biomedical and clinical research, particularly in the analysis of chiral drugs in biological matrices (Chan et al., 2016).

  • High-performance liquid chromatographic enantioseparation of 2-aminomono- and dihydroxycyclopentanecarboxylic and 2-aminodihydroxycyclohexanecarboxylic acids on macrocyclic glycopeptide-based phases.: This research focuses on the enantioseparation of various amino acids using the Astec CHIROBIOTIC T Chiral HPLC Column, highlighting its effectiveness in separating complex chiral molecules. The study underscores the column′s application in the enantioselective analysis of amino acids and related compounds in chemical and pharmaceutical research (Berkecz et al., 2009).

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Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

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Balázs Visegrády et al.
Journal of biochemical and biophysical methods, 53(1-3), 15-24 (2002-10-31)
This contribution deals with comparative studies on the chiral separation of thiazide diuretics using cellulose tris(3,5-dimethylphenylcarbamate) (Chiralcel OD-RH), cellulose tris(4-methylbenzoate) (Chiralcel OJ-R) and teicoplanin (Chirobiotic T) phases. All columns showed good chiral recognition ability for this class of compounds. Out
Felikss Mutulis et al.
Bioorganic & medicinal chemistry, 15(17), 5787-5810 (2007-07-10)
Two hundred and ten tertiary amides were prepared on solid phase. Diamines were coupled to activated carboxylated Wang polymer, and the polymeric substituted benzyloxycarbonyl protected diamines obtained were reacted with aldehydes or ketones in trimethyl orthoformate giving resin attached Schiff
Zahid Ali et al.
Journal of chromatography. A, 1052(1-2), 199-204 (2004-11-06)
The solvation parameter model is used to characterize the retention properties of a 3-aminopropylsiloxane-bonded (Alltima amino), three 3-cyanopropylsiloxane-bonded (Ultrasphere CN, Ultremex-CN and Zorbax SB-CN), a spacer bonded propanediol (LiChrospher DIOL) and a multifunctional macrocyclic glycopeptide (Chirobiotic T) silica-based stationary phases
B Mistry et al.
Journal of chromatography. B, Biomedical sciences and applications, 758(2), 153-161 (2001-08-07)
A sensitive and stereoselective high-performance liquid chromatographic assay for the determination of the enantiomers of metoprolol (R- and S-) and the diastereoisomers of alpha-hydroxymetoprolol (IIA, IIB) in plasma is reported. Chromatography involved direct separation of enantiomers using a Chirobiotic T
Yuan-Qing Xia et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 788(2), 317-329 (2003-04-23)
An automated online sample extraction method for rat plasma was developed and validated for the quantification of (R)- and (S)-propranolol following the intravenous administration of either the racemate or the individual enantiomers at 5 mg/kg. A dual-column extraction system coupled

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