Skip to Content
Merck
All Photos(1)

Documents

T4319

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, liquid

Synonym(s):

TMB

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

product name

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Slow Kinetic Form, for ELISA, peroxidase substrate

Quality Level

300

form

liquid

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Application

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Slow Kinetic Form, for ELISA has been used as a substrate for anti-bovine IgG-peroxidase conjugated antibody and for horse radish peroxidase-conjugated mouse anti-mouse IgG in Poly (ADP-ribose) binding assays.
Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 25% slower than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protection of cattle against a natural infection of Fasciola hepatica by vaccination with recombinant cathepsin L1 (rFhCL1)
Golden O, et al.
Vaccine, 28(34), 5551-5557 (2010)
Efficient single-strand break repair requires binding to both poly (ADP-Ribose) and DNA by the central BRCT domain of XRCC1
Polo LM, et al.
Testing, 26(3), 573-581 (2019)
Luis M Polo et al.
Cell reports, 26(3), 573-581 (2019-01-17)
XRCC1 accelerates repair of DNA single-strand breaks by acting as a scaffold protein for the recruitment of Polβ, LigIIIα, and end-processing factors, such as PNKP and APTX. XRCC1 itself is recruited to DNA damage through interaction of its central BRCT

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service