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Key Documents

SAB4200688

Sigma-Aldrich

Anti-Bovine Serum Albumin antibody, Mouse monoclonal

clone BSA-33, purified from hybridoma cell culture

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

BSA-33, monoclonal

form

buffered aqueous solution

mol wt

antigen ~70 kDa

species reactivity

sheep (highly reactive ), horse (less reactive ), dog (less reactive ), turkey (less reactive ), goat (highly reactive )

concentration

~1 mg/mL

technique(s)

immunoblotting: 0.5-1 μg/mL using Fetal Bovine Serum.
immunoprecipitation (IP): 1-2 μg using Fetal Bovine Serum.
indirect ELISA: 0.03-0.075 μg/mL using 5 μg/mL BSA for coating.

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

dog ... Alb(403550)
horse ... Alb(100034206)
sheep ... Alb(443393)

General description

Serum albumin, the most abundant protein (30–50 mg/mL) in the plasma, is a multifunctional, non-glycosylated, negatively charged protein produced in the liver. It binds to a wide variety of lipophilic compounds such as metal ions, pharmaceuticals and metabolites. Albumin regulates osmotic pressure in the blood and has a role in the transport of fatty acids.

Immunogen

Bovine Serum Albumin (BSA)

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Albumin: Biochemical properties and therapeutic potential*
Gregory J. Quinlan, et al.
Hepatology (2005)
Clinical use of albumin in hepatology
Matteo G, et al.
Blood Transfusion = Trasfusione del Sangue, 7(4), 268-277 (2009)
Tatsat Banerjee et al.
Nature communications, 14(1), 7909-7909 (2023-12-01)
The plasma membrane is widely regarded as the hub of the numerous signal transduction activities. Yet, the fundamental biophysical mechanisms that spatiotemporally compartmentalize different classes of membrane proteins remain unclear. Using multimodal live-cell imaging, here we first show that several
Spatiotemporal dynamics of membrane surface charge regulates cell polarity and migration.
Banerjee, et al.
Nature Cell Biology, 24, 1499-1515 (2023)

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