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Key Documents

SAB1305415

Sigma-Aldrich

ANTI-MLCK(N-TERMINAL) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Myosin light chain kinase

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

210715 Da

species reactivity

human, mouse

technique(s)

immunohistochemistry: 1:50-1:100
western blot: 1:1000

NCBI accession no.

NP_444253.3

UniProt accession no.

Q15746

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYLK(4638)

General description

Myosin light chain kinase (MLCK) is a serine/threonine kinase and the gene encoding it is localized on human chromosome 3q21.1.

Biochem/physiol Actions

Myosin light chain kinase (MLCK) is activated by the binding of Ca2+/calmodulin. It has a role in smooth muscle contraction. The kinase phosphorylates the regulatory light chain of smooth muscle myosin. This stimulates ATPase activity of the myosin heads and leads to the myosin power stroke, which is crucial for muscle contraction. MLCK also assists the interaction of myosin with actin. Loss of function of the protein has been linked to megacystis microcolon intestinal hypoperistalsis syndrome.

Physical form

Supplied in PBS with 0.09% (W/V) sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Loss-of-Function Variants in MYLK Cause Recessive Megacystis Microcolon Intestinal Hypoperistalsis Syndrome.
Halim D
American Journal of Human Genetics (2017)
A novel variant in MYLK causes thoracic aortic dissections: genotypic and phenotypic description.
Hannuksela M
BMC Medical Genetics (2016)
Increasing evidence of mechanical force as a functional regulator in smooth muscle myosin light chain kinase.
Baumann F
eLife (2017)
Bingqing Bai et al.
Molecular medicine reports, 25(4) (2022-02-10)
Aberrant TGF‑β/Smad7 signaling has been reported to be an important mechanism underlying the pathogenesis of ulcerative colitis. Therefore, the present study aimed to investigate the effects of a number of potential anti‑colitis agents on intestinal epithelial permeability and the TGF‑β/Smad7

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