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Key Documents

PLA0079

Sigma-Aldrich

Rabbit anti-PCNA Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

Synonym(s):

ATLD2, DNA polymerase delta auxiliary protein, PCNA, cyclin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

grade

Powered by Bethyl Laboratories, Inc.

species reactivity

human, mouse

technique(s)

flow cytometry: 0.03 μg
immunohistochemistry: 1:2,000- 1:10,000
immunoprecipitation (IP): 2- 5 μg/mg
western blot: 1:1,000- 1:10,000

accession no.

P12004

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

rabbit ... PCNA(5111)

General description

Proliferating cell nuclear antigen (PCNA), a single-copy gene, spans about 12 kb. This gene is located on human chromosome 20p13. PCNA is expressed at high levels in the thymus, bone marrow, fetal liver, and some cells of the small intestine and colon. It is located in the nucleus.

Immunogen

The epitope recognized by PLA0079 maps to a region between residues 75 and 125 of human proliferating cell nuclear antigen using the numbering given in SwissProt entry P12004 (GeneID 5111).

Application

Rabbit anti-PCNA Antibody, Affinity Purified has been used in:
  • proximity ligation assay (PLA) (1:500)
  • immunoblotting (1:2000)
  • flow cytometry analysis

Biochem/physiol Actions

Proliferating cell nuclear antigen (PCNA) protein functions as an auxiliary factor of polymerase δ. It acts as a cofactor to polymerase ε, polymerase β, and translesion synthesis polymerases, such as η, κ, λ, and θ. In chronic lymphoid leukemia (CLL) patients, intracellular levels of PCNA protein can be used as a marker to identify clinical behavior and survival.

Physical form

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Other Notes

PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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PCNA on the crossroad of cancer
Stoimenov I, Helleday T
Atlas of Genetics and Cytogenetics in Oncology and Haematology (2009)
Qun Wang et al.
Heart, lung & circulation, 25(11), 1124-1132 (2016-05-18)
Coronary artery bypass grafting is employed for the treatment of multiple-vessel lesions. Studies have shown that continued maladaptive remodelling expedites atherosclerosis and that excessive remodelling leads to graft restenosis. However, few studies have focussed on mechanistic target of rapamycin (mTOR)
Qiyuan Zhou et al.
American journal of respiratory cell and molecular biology, 54(5), 728-739 (2015-10-22)
We have reported that von Hippel-Lindau protein (pVHL) expression is elevated in human and mouse fibrotic lungs and that overexpression of pVHL stimulates fibroblast proliferation. We sought to determine whether loss of pVHL in fibroblasts prevents injury and fibrosis in
James P Wells et al.
PLoS genetics, 18(12), e1010309-e1010309 (2022-12-09)
DNA replication is a vulnerable time for genome stability maintenance. Intrinsic stressors, as well as oncogenic stress, can challenge replication by fostering conflicts with transcription and stabilizing DNA:RNA hybrids. RAD18 is an E3 ubiquitin ligase for PCNA that is involved
Emily Yun-Chia Chang et al.
Nature communications, 10(1), 4265-4265 (2019-09-21)
Ectopic R-loop accumulation causes DNA replication stress and genome instability. To avoid these outcomes, cells possess a range of anti-R-loop mechanisms, including RNaseH that degrades the RNA moiety in R-loops. To comprehensively identify anti-R-loop mechanisms, we performed a genome-wide trigenic

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