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P5119

Sigma-Aldrich

Phosphate buffered saline

liquid, pH 7.2 (25 °C)

Synonym(s):

PBS

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About This Item

MDL number:
UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25
Pricing and availability is not currently available.

Product Name

Phosphate buffered saline, pH 7.2 (25 °C)

sterility

autoclaved

Quality Level

form

liquid

pH

7.2 (25 °C)

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

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General description

Phosphate buffered saline (PBS) is an isotonic buffer solution. This water-based salt solution is made up of disodium hydrogen phosphate, sodium chloride and, in some preparations, potassium chloride and potassium dihydrogen phosphate. PBS is generally used in biological research.[1]

Application

It has also been used for the dilution of capture antibody during α-hemolysin (Hla) enzyme-linked immunosorbent assay (ELISA).[2]
Phosphate buffered saline has been used in bis-benzimide accumulation assay.[3]

Components

Sodium chloride, 145 mM (0.85%) in phosphate buffer, 150 mM

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Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Experimental? review? of? cobalt? induced cardiomyopathy
Zadnipryany I, et al.
Russian Open Medical Journal, 6(1), 103-103 (2017)
Measuring the activity of active efflux in Gram-negative bacteria
Antibiotic resistance protocols, 173-180 (2010)
ELISA for Alpha-hemolysin (Hla) in Methicilin-resistant Staphylococcus aureus (MRSA)
Khodaverdian VY and Shoham M
Antimicrobial agents and chemotherapy, 6 (2013)
Daniela Michlmayr et al.
Cell reports, 31(4), 107569-107569 (2020-04-30)
Zika virus (ZIKV) is an emerging, mosquito-borne flavivirus responsible for recent epidemics across the Americas, and it is closely related to dengue virus (DENV). Here, we study samples from 46 DENV-naive and 43 DENV-immune patients with RT-PCR-confirmed ZIKV infection at
Yong N Li et al.
The Journal of comparative neurology, 516(5), 442-453 (2009-08-06)
Horizontal cells (HCs) are involved in establishing the center-surround receptive field organization of photoreceptor and bipolar cells. In many species, HCs respond differentially to colors and may play a role in color vision. An earlier study from our laboratory suggested

Questions

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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