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L8912

Sigma-Aldrich

L-Leucine

98.5-101.0%, suitable for cell culture, non-animal source, meets EP, JP, USP testing specifications

Synonym(s):

(S)-Leucine, (S)-2-Amino-4-methylpentanoic acid

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About This Item

Linear Formula:
(CH3)2CHCH2CH(NH2)CO2H
CAS Number:
Molecular Weight:
131.17
Beilstein:
1721722
EC Number:
MDL number:
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.26
Pricing and availability is not currently available.

Product Name

L-Leucine, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%

biological source

non-animal source

Agency

USP/NF
meets EP testing specifications
meets JP testing specifications
meets USP testing specifications

Assay

98.5-101.0%

form

powder

optical activity

[α]20/D 14.9 to 16.0°, c = 4 in 6 M HCl
[α]25/D 14.9 to 17.3 °, c = 4 in 6 M HCl

quality

meets EP, JP, USP testing specifications

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

mp

>300 °C (lit.)

solubility

1 M HCl: 50 mg/mL

cation traces

As: ≤1 ppm
Fe: ≤10 ppm
NH4+: <0.02%
heavy metals: ≤10 ppm

application(s)

peptide synthesis
pharmaceutical (small molecule)

functional group

amine
carboxylic acid

storage temp.

room temp

SMILES string

CC(C)C[C@H](N)C(O)=O

InChI

1S/C6H13NO2/c1-4(2)3-5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t5-/m0/s1

InChI key

ROHFNLRQFUQHCH-YFKPBYRVSA-N

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General description

Leucine is a non-glucogenic, and essential amino acid. It is a branched-chain amino acid that is a structural component of proteins.[1]

Application

L-Leucine has been used:

  • as a hypertrophic agent to monitor its effects on myotubes.[2]
  • as a component of PMG media for culturing yeast.[3]
  • in myotube protein synthesis analysis in skeletal muscle cells.[4]
  • to study its effects on hepatic lipid metabolism.[5]

Biochem/physiol Actions

Leucine plays a role in intracellular signaling, gene transcription, and translation. It promotes the synthesis of protein by activating the mammalian target of the rapamycin (mTOR) signaling pathway in placental cells, skeletal muscles, and adipose tissue.[6] Leucine inhibits protein degradation and promotes mitochondrial biogenesis, glucose metabolism, and fatty acid oxidation to provide energy for protein synthesis.[1]

Other Notes

For additional information on our range of Biochemicals, please complete this form.
The product is intended for laboratory use only, and it is not suitable for drug, household, or other purposes.

comparable product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

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Optimization of an in vitro bioassay to monitor growth and formation of myotubes in real time
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Bioscience Reports (2016)
(-)-Epicatechin and its colonic metabolite hippuric acid protect against dexamethasone-induced atrophy in skeletal muscle cells
Edwards S J, et al.
The Journal of Nutritional Biochemistry, 110, 109150- 109150 (2022)
The role of leucine and its metabolites in protein and energy metabolism
Duan Y, et al.
Amino Acids, 48(1), 41-51 (2016)
Leucine in Obesity: Therapeutic Prospects
Yao K, et al.
Trends in Pharmacological Sciences, 37(8), 714-727 (2016)
Yeast Augmented Network Analysis (YANA): a new systems approach to identify therapeutic targets for human genetic diseases
David J
F1000Research (2014)

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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