G1924
L-Glutamate Oxidase from Streptomyces sp.
recombinant, expressed in E. coli
Synonym(s):
L-Glutamate:oxygen oxidoreductase (deaminating)
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About This Item
Recommended Products
recombinant
expressed in E. coli
Quality Level
form
solid
specific activity
≥5 units/mg protein
mol wt
120 kDa
storage temp.
−20°C
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General description
L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.
This product has a 6xHistidine tag with a thrombin cleavage site.
Application
L-Glutamate Oxidase from Streptomyces sp may be used:
- as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor development
- as a component of pencil graphite electrode for biosensing L-glutamate
Biochem/physiol Actions
L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.
Unit Definition
One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Imidazole glycerol phosphate synthase (ImGPS) is an allosteric bienzyme complex in which substrate binding to the synthase subunit HisF stimulates the glutaminase subunit HisH. To control this stimulation with light, we have incorporated the photo-responsive unnatural amino acids phenylalanine-4'-azobenzene (AzoF)
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