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Key Documents

E8406

Sigma-Aldrich

Anti-Parvalbumin Antibody

rabbit polyclonal

Synonym(s):

Anti-EDEM, Anti-ER degradation enhancer, mannosidase alpha-like 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

Pricing and availability is not currently available.

product name

Anti-EDEM1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 65 kDa (nonglycosylated human and mouse EDEM1)
antigen 75 kDa

species reactivity

human, mouse

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HPA003245MABN2620ZRB1098
antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified antibody

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

200

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

ambient

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:20-1:50

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable (peptide)

species reactivity

fish, frog, mouse, human

species reactivity

human

species reactivity

human, mouse, rat

species reactivity

rat, human, mouse

General description

ER degradation-enhancing alpha-mannosidase-like 1 (EDEM1) is an enzyme encoded by the EDEM1 gene in humans. In mammalian cells, EDEM1 is localized to the endoplasmic reticulum (ER), mainly as a soluble glycoprotein.
glycoslylated human and mouse EDEM1

Immunogen

synthetic peptide corresponding to amino acids 629-646 of human EDEM1, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence is identical in mouse EDEM1.

Application

Anti-EDEM1 antibody has been used in immunoblotting.
Anti-EDEM1 antibody produced in rabbit is suitable for the following applications:
  • Immunoprecipitation at a concentration of 0.5-1.0μg using extract HEK-293T cells expressing recombinant mouse EDEM1 protein.
  • Western blotting at a concentration of 0.5-1.0μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM1.
  • Immunofluorescence Microscopy.
  • Immunoblotting
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

ER degradation-enhancing alpha-mannosidase-like 1 (EDEM1), a putative mannose binding lectin, targets misfolded glycoproteins for degradation in an N-glycan dependent manner. It interacts with the C-terminus of calnexin and lacks mannosidase activity.
ER degradation-enhancing alpha-mannosidase-like 1 is an enzyme encoded by the EDEM1 gene in humans. It is a chaperone for rod opsin and its expression can be used to promote correct folding enhancing degradation of mutant proteins in the ER to combat protein-misfolding disease. The endogenous EDEM1 in cells that are not stressed by the expression of a transgenic misfolded protein reaches the cytosol and is degraded by basal autophagy. EDEM may maintain the retrotranslocation competence of NHK by inhibiting aggregation. It is also considered responsible for directing aberrant proteins for ERAD (ER-associated protein degradation).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

  • Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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    Redundant and antagonistic roles of XTP3B and OS9 in decoding glycan and non-glycan degrons in ER-associated degradation
    van der Goot A T, et al.
    Molecular Cell, 70, 516-530 (2018)
    EDEM1 recognition and delivery of misfolded proteins to the SEL1L-containing ERAD complex
    Cormier J H, et al.
    Molecular Cell, 34(5), 627-633 (2009)
    Sujin Park et al.
    Histochemistry and cell biology, 142(2), 153-169 (2014-03-26)
    ER degradation-enhancing α-mannosidase-like 1 protein (EDEM1) is involved in the routing of misfolded glycoproteins for degradation in the cytoplasm. Previously, we reported that EDEM1 leaves the endoplasmic reticulum via non-COPII vesicles (Zuber et al. in Proc Natl Acad Sci USA
    Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation
    van der Goot AT, et al.
    Molecular Cell, 70(3), 516-530 e6-516-530 e6 (2018)
    Iryna Monastyrska et al.
    Autophagy, 9(2), 164-174 (2012-11-28)
    Equine arteritis virus (EAV) is an enveloped, positive-strand RNA virus. Genome replication of EAV has been associated with modified intracellular membranes that are shaped into double-membrane vesicles (DMVs). We showed by immuno-electron microscopy that the DMVs induced in EAV-infected cells

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