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64958

Sigma-Aldrich

2-Methoxy-2,4-diphenyl-3(2H)-furanone

suitable for fluorescence, ≥98.0% (HPLC)

Synonym(s):

2,4-Diphenyl-2-methoxy-3(2H)-furanone, MDPF

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About This Item

Empirical Formula (Hill Notation):
C17H14O3
CAS Number:
50632-57-0
Molecular Weight:
266.29
Beilstein:
1288529
MDL number:
MFCD00036834
UNSPSC Code:
12352108
PubChem Substance ID:
329760016
NACRES:
NA.32

Quality Level

100

Assay

≥98.0% (HPLC)

form

solid

fluorescence

λex 384 nm; λem 472 nm in acetonitrile (after derivatization with hexylamine)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

COC1(OC=C(C1=O)c2ccccc2)c3ccccc3

InChI

1S/C17H14O3/c1-19-17(14-10-6-3-7-11-14)16(18)15(12-20-17)13-8-4-2-5-9-13/h2-12H,1H3

InChI key

BLWINLJDTOJSRU-UHFFFAOYSA-N

Application

2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) is used as a fluorescence reagent to derivatize primary and secondary amines on molecules such as proteins. MDPF derivatized molecules can be detected during analytical and separation procedures including chromatography, electrophoresis and zymography.

Other Notes

Reagent for the derivatization of primary and secondary amines for HPLC; highly fluorescent derivatives are formed; Pre-column derivatization of amines; Fluorescent labeling of proteins before SDS-PAGE

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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V E Urwin et al.
Analytical biochemistry, 195(1), 30-37 (1991-05-15)
A multiple mini two-dimensional electrophoretic method which results in three two-dimensional protein spot patterns being positioned side by side in an individual gel has been developed. Preparation time has been minimized by employing disposable capillary tubes for the isoelectric focusing
Pablo Castro-Hartmann et al.
Electrophoresis, 25(15), 2501-2505 (2004-08-10)
The high-energy intermediates generated in the reaction of bis(2,4,6-trichlorophenyl)oxalate (TCPO) with H2O2 can excite electronically different fluorophores with a high quantum yield in organic solvents. We have previously applied this peroxyoxalate chemiluminescent reaction to the detection of proteins labeled with
S. Stein
Pept. Neurobiol., 9-9 (1977)
V Toome et al.
Biochemical and biophysical research communications, 114(2), 433-439 (1983-07-29)
2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) reacts readily with the free amino group of a dipeptide to form a pyrrolinone-type chromophore with absorption maxima at 275-285 and 370-390 nm. A simple test tube procedure is described which allows in situ correlation of the absolute
Karen Martin et al.
Proteomics, 3(7), 1215-1227 (2003-07-23)
Three-color fluorescence detection methods are described based upon covalently coupling the dye 2-methoxy-2,4-diphenyl-2(2H)-furanone (MDPF) to proteins immobilized on poly(vinylidene difluoride) (PVDF) membranes, followed by detection of target proteins using alkaline-phosphatase-conjugated reporter molecules in combination with the fluorogenic substrate 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) phosphate
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