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T3260

Sigma-Aldrich

Toluidine Blue O

Powder

Synonym(s):

Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride

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About This Item

Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
92-31-9
Molecular Weight:
305.83
Colour Index Number:
52040
EC Number:
202-146-2
MDL number:
MFCD00011934
UNSPSC Code:
12171500
PubChem Substance ID:
24900122
NACRES:
NA.47
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Product Name

Toluidine Blue O, Technical grade

grade

technical grade

Quality Level

200

form

powder

composition

Dye content, ~80%

technique(s)

microbe id | staining: suitable

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C

InChI

1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H

InChI key

GEDVVYWLPUPJJZ-UHFFFAOYSA-N

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General description

A metachromatic dye suitable for a wide variety of histological staining procedures.
Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. It is a metachromatic dye suitable for a wide variety of histological staining procedures.[1][2]

Application

Toluidine Blue O has been used to stain a variety of specimens like mesenchymal cells, taste buds, osteoblasts, matrix, etc.[3][4][5][6]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR9627
0000289415
SHBQ6029
0000231220
SHBL9130

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Mrinalini Sharma et al.
Antimicrobial agents and chemotherapy, 52(1), 299-305 (2007-10-31)
Staphylococci are important causes of nosocomial and medical-device-related infections. Their virulence is attributed to the elaboration of biofilms that protect the organisms from immune system clearance and to increased resistance to phagocytosis and antibiotics. Photodynamic treatment (PDT) has been proposed
Polychromatic staining of plant cell walls by toluidine blue O
T.P.O'Brien
Protoplasma, 59(2) (1964)
The canine epiphyseal-derived mesenchymal stem cells are comparable to bone
marrow derived-mesenchymal stem cells
Ya-Pei CHANG
Surgery (2015)
Fibroblast-derived matrix (FDM) as a novel vascular endothelial growth factor delivery platform
Ping Du
Journal of Controlled Release : Official Journal of the Controlled Release Society (2014)
An investigation of cellular dynamics during the development of intramembranous bones: the scleral ossicles
J. Jabalee
Journal of Anatomy (2013)
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Questions

1–3 of 3 Questions  

  1. Are there any instructions on how to mix Toluidine Blue O (PN T3260) into a staining solution?

    1 answer

    1. Depending on the staining target, the preparation of the dye solution can vary. One method involves dissolving the dye at 0.01% in water. Other method uses a 2% concentration in water. For mast cells, another procedure involves using 0.02 grams of the dye, dissolving it in 100 ml of water, and adding 0.25ml of glacial acetic acid. In electron microscopy, two solutions are made: Solution 1 (2 grams of dye dissolved in 100 ml of water) and Solution 2 (2 grams of sodium borate, also dissolved in water). Equal parts of Solution 1 and Solution 2 are used for staining. Not all lots of dye are soluble to 2% in water. T3260 is tested for solubility at 4mg of dye in 4 ml of water, or 1 mg/ml. Point to note is that whether it will provide adequate staining needs to be determined since it hasnot been involved in staining actual cells or tissue sections.

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  2. Is there a difference between Toluidine Blue O and Toluidine Blue and which is best for staining tissue sections?

    1 answer

    1. Toluidine Blue O and Toluidine Blue are generally regarded as synonyms. The dye can be found in 2 forms. There is a chloride form and zinc chloride double salt.  Products T3260 and 198161 are the chloride form. This is the form certified by the Biological Stain Commission. The dye listed as simply Toluidine Blue, Product 89640, is the zinc chloride double salt. For routine use today, the chloride form should be utilized. The product number recommended for biological/histological staining is Product 198161, Toluidine Blue O.  This product has been found to provide acceptable staining of tissue by the Biological Stain Commission. Products 198161 and 89640 have not been suitability tested for the staining of tissue sections.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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