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Key Documents

I8377

Sigma-Aldrich

Iodonitrotetrazolium chloride

Used in colorimetric assays.

Synonym(s):

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT

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About This Item

Empirical Formula (Hill Notation):
C19H13ClIN5O2
CAS Number:
Molecular Weight:
505.70
Beilstein:
4093224
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

Quality Level

Assay

≥98% (TLC)

form

powder

mp

240 °C (dec.) (lit.)

solubility

H2O: 4 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4

InChI

1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1

InChI key

JORABGDXCIBAFL-UHFFFAOYSA-M

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Application

Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.
Electron acceptor for the colorimetric assay of various dehydrogenases

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Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Flam. Sol. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

4.1B - Flammable solid hazardous materials

WGK

WGK 3


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Ignatoski KMW and Ethier SP
Breast Cancer Research and Treatment, 54, 173-173 (1999)
Ruiwu Wang et al.
Insect biochemistry and molecular biology, 33(7), 733-739 (2003-06-27)
Voltage-gated sodium channels are essential for the generation and propagation of action potentials in most excitable cells. They are the target sites of several classes of insecticides and acaricides. Isolation of full-length sodium channel cDNA is a critical and often
Yang Hongwei et al.
Ecotoxicology and environmental safety, 53(3), 416-421 (2002-12-18)
This study assessed anaerobic biodegradability of organic compounds from microorganism activity. Dehydrogenase activity can be a good parameter characterizing the microorganism activity. A modified method of 2-(p-iodophenyl-3-(p-nitrophenyl)-5-pheny tetrazolium chloride-dehydrogenase activity determination was proposed in anaerobic biodegradability assessment. Cubic spline curves
T J Mankelow et al.
The Biochemical journal, 358(Pt 2), 315-324 (2001-08-22)
Diethyl pyrocarbonate (DEPC), a histidine-modifying reagent, has been utilized to demonstrate the importance of histidine residues in the functioning of proteins. In previous studies of the NADPH oxidase, histidine residues have been determined to be important in the ability of
Cheng Luo et al.
Journal of biochemical and biophysical methods, 68(2), 101-111 (2006-06-03)
A sensitive spectrophotometric assay for determining mitochondrial malate dehydrogenase activity is described. The assay measures NADH production by coupling it to the reduction of 2-(p-iodophenyl)-3(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT). Via an intermediate electron carrier, either phenazine methosulfate or lipoamide dehydrogenase, INT

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