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317802

Sigma-Aldrich

4-(4-Isothiocyanatophenylazo)-N,N-dimethylaniline

97%

Synonym(s):

4-(Dimethylamino)azobenzene-4′-isothiocyanate, DABITC

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About This Item

Linear Formula:
SCNC6H4N=NC6H4N(CH3)2
CAS Number:
7612-98-8
Molecular Weight:
282.36
Beilstein:
752568
EC Number:
231-521-3
MDL number:
MFCD00004812
UNSPSC Code:
12171500
PubChem Substance ID:
24859100
NACRES:
NA.47

Assay

97%

form

crystals

mp

167-171 °C (lit.)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CN(C)c1ccc(cc1)\N=N\c2ccc(cc2)N=C=S

InChI

1S/C15H14N4S/c1-19(2)15-9-7-14(8-10-15)18-17-13-5-3-12(4-6-13)16-11-20/h3-10H,1-2H3/b18-17+

InChI key

OSWZKAVBSQAVFI-ISLYRVAYSA-N

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKBC7832
14127JI
12029JC
09630CC
06912HO

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R Aebersold et al.
Analytical biochemistry, 136(2), 465-469 (1984-02-01)
Proteins and polypeptides are derivatized with dimethylaminoazobenzene isothiocyanate (DABITC) before their separation on sodium dodecyl sulfate-polyacrylamide gels. DABITC-derivatized proteins are detected visually in the picomole range without further staining and destaining procedures. The recovered colored protein can also be used
P M Fischer et al.
Analytical biochemistry, 177(1), 46-49 (1989-02-15)
A single-column high-performance liquid chromatographic separation of 4-N,N-dimethylaminoazobenzene 4'-thiohydantoin amino acid derivatives, generated during polypeptide sequence analysis by the 4-N,N-dimethylaminoazobenzene 4'-isothiocyanate/phenylisothiocyanate double coupling technique, is described. Recovery of the serine and threonine derivatives was improved by substituting boron trifluoride-diethyl etherate
A S Babu et al.
Toxicon : official journal of the International Society on Toxinology, 29(10), 1251-1262 (1991-01-01)
The effects of chemical modification with 4-NN-dimethyl amino azo benzene-4'-isothiocyanate on various biological activities of phospholipases A2, NN-XIII-PLA2 from Naja naja naja and VRV-PL-VIIIa from Vipera russelli snake venoms were investigated. Modification of the enzymes resulted in significant reduction of
I Jelesarov et al.
European journal of biochemistry, 216(1), 57-66 (1993-08-15)
The chloroplast enzyme ferredoxin: NADP+ reductase (FNR) catalyzes the reduction of NADP+ by ferredoxin (Fd). FNR and Fd form a 1:1 complex that is stabilized by electrostatic interactions between acidic residues of Fd and basic residues of FNR. To localize
J Y Chang et al.
Biochemistry, 31(11), 2874-2878 (1992-03-24)
Recombinant human interleukin-1 beta (h-IL-1 beta) was chemically modified with 4-(N,N-dimethylamino)-4'-isothiocyanatoazobenzene-2'-sulfonic acid (S-DABITC), a water-soluble color reagent specific for lysine labeling. Modified h-IL-1 beta was digested by lysyl endopeptidase. Peptides containing labeled lysines were detected at the visible wavelength (436
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