The recommended working concentration for cell lysate application is 1 mM. It's important to note that orthovanadate can be toxic in the blood at a concentration of 0.12 mM. For further information, you can refer to the publication "Biochim Biophys Acta. 2012 Sep;1818(9):2260-70" with the PMID: 22546530. This publication discusses the effects of sodium orthovanadate on human erythrocytes and neuroblastoma cells in vitro. It mentions that the lethal concentration of vanadium in the blood of a patient was calculated to be about 0.12 mM.
5.08605
Sodium Orthovanadate, Activated
Synonym(s):
Sodium Orthovanadate, Activated, Orthovanadate, Activated, Vanadate, Activated
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About This Item
Empirical Formula (Hill Notation):
Na3O4V
Molecular Weight:
183.91
UNSPSC Code:
12352202
NACRES:
NB.21
Pricing and availability is not currently available.
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form
liquid
Quality Level
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
protect from light
color
colorless
storage temp.
−70°C
General description
A depolymerized vanadate prepared from Sodium Orthovanadate (>Cat. No. 567540) that exhibits significant enhanced ability to inhibit endogenous phosphatases in a competitive manner. Preserves the phosphorylation state in cells and cell lysates. Completely reversible with EDTA and dilution. Recommended concentration in lysis buffer ~ 1 mM. Available as 5 ml or as a set of 3 x 1 ml.
Biochem/physiol Actions
Primary Target
Phosphatases
Phosphatases
Reversible: yes
Packaging
Packaged under inert gas
Warning
Toxicity: Toxic (F)
Physical form
Supplied as 10 mg/ml in aqueous NaOH, pH 10.0.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.
Other Notes
Gordon, J.A. 1984. Methods. Enzymol.201, 477.
Brown, D.J., and Gordon, J.A. 1984. J. Biol. Chem.259, 9580.
Brown, D.J., and Gordon, J.A. 1984. J. Biol. Chem.259, 9580.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
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