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recombinant
expressed in E. coli
Assay
70-95% (SDS-PAGE)
form
frozen liquid
mol wt
~39 kDa
concentration
0.2 μg/μL
accession no.
P10636-3
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MAPT(4137)
Application
Useful for kinase assay and Western blot.
Biochem/physiol Actions
Recombinant full-length human tag-free Tau-316 was expressed in E. coli cells. Tau-316 encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. Tau-316 transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type (1). Tau-316 gene mutations have been associated with several neurodegenerative disorders such as Alzheimer′s disease, Pick′s disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy (2).
Sequence
Full Length
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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