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RAB0331

Sigma-Aldrich

Human PSA-total ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

Gamma-seminoprotein), Kallikrein-3, Prostate-specific antigen

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type urine
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL
standard curve range: 10.24-2500 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... KLK3(354)

General description

The Human PSA-total (Prostate Specific Antigen, total) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme- linked immunosorbent assay for the quantitative measurement of human PSA-total in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human PSA

Application

For research use only. Not for use in diagnostic procedures.
Human PSA-total ELISA Kit has been used to determine PSA (prostate specific antigen) level in serum of prostate cancer patients and cancer-free patients.

Biochem/physiol Actions

Prostate-specific antigen (PSA) is an androgen-regulated serine protease specifically produced by prostatic tissue and is secreted into the lumen. PSA in semen cleaves semenogelins present in the seminal coagulum. Total PSA levels are increased in serum of prostate cancer (PCa) patients. PSA functions as a potential biomarker for PCa. Serum protease inhibitors such as α1-antichymotrypsin (α1-ACT), α2-macroglobulin (α2-M), and other acute-phase proteins form irreversible complexes with circulating PSA and hinder proteolytic activity of PSA.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Prostate-specific antigen: biochemistry, analytical methods, and clinical application.
Armbruster DA
Clinical Chemistry, 39(2), 181-195 (1993)
André Sarmento-Cabral et al.
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Karteek Kadimisetty et al.
Analytical methods : advancing methods and applications, 10(32), 4000-4006 (2019-03-25)
Low cost, miniaturized assay platforms that work with small sample volumes, high sensitivity and rapid detection will have high value in future biomolecular diagnostics. Herein we report an automated, 3D printed electrochemiluminescent (ECL) immunoarray integrated with a nanostructured pyrolytic graphite
C K Tang et al.
Lab on a chip, 17(3), 484-489 (2017-01-10)
A low cost three-dimensional (3D) printed clear plastic microfluidic device was fabricated for fast, low cost automated protein detection. The unibody device features three reagent reservoirs, an efficient 3D network for passive mixing, and an optically transparent detection chamber housing
Mohamed Sharafeldin et al.
Biosensors & bioelectronics, 91, 359-366 (2017-01-06)
Ultrasensitive mediator-free electrochemical detection for biomarker proteins was achieved at low cost using a novel composite of Fe3O4 nanoparticles loaded onto graphene oxide (GO) nano-sheets (Fe3O4@GO). This paramagnetic Fe3O4@GO composite (1µm size range) was decorated with antibodies against prostate specific
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