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Key Documents

RAB0028

Sigma-Aldrich

Human BMP2 ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

BMP-2, Bone morphogenetic protein 2

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

Pricing and availability is not currently available.

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type cell culture supernatant(s)
sample type urine
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 45 pg/mL
standard curve range: 46.88-3000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... BMP2(650)

General description

BMP2 (bone morphogenetic protein 2) is encoded by the gene mapped to human chromosome 20p12.3.[1] The encoded protein belongs to transforming growth factor-β (TGFβ) superfamily. BMP-2 is expressed in heart, lung, and dorsal aorta.[2]
The Human BMP-2 ( Bone morphogenetic proteins-2) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme- linked immunosorbent assay for the quantitative measurement of human BMP-2 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human BMP2

Application

For research use only. Not for use in diagnostic procedures.
Human BMP2 (bone morphogenetic protein 2) ELISA kit has been used for measuring concentrations of BMP in conditioned mouse organoid media.[3]
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

BMP2 (bone morphogenetic protein 2) plays a vital role in the activation of the autonomic lineage-specific basic–helix-loop-helix protein MASH1 (mammalian achaete scute homolog-1) and in the autonomic neurogenesis in neural crest stem cells in vitro.[2] During bone repair, BMP-2 controls the expression of Runt-related transcription factor 2 (Runx2) and Osterix (Osx), thereby enhancing migration, proliferation and osteogenic differentiation of mesenchymal stem cells.[4] In addition to its osteogenic activity, BMP-2 also plays an important role in cardiac morphogenesis.[5] Recombinant human bone morphogenetic protein-2 (rhBMP-2) plays an essential role in pathogenesis of oral squamous cell carcinomas.[6] Mutation in BMP-2 gene is associated with osteoporosis.[1]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials


  • Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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