P6486
EZview™ Red Protein A Affinity Gel
Synonym(s):
EZview Affinity Gel, Protein A Affinity Gel
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About This Item
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form
liquid
analyte chemical class(es)
proteins (Immunoglobulins of various mammalian species)
technique(s)
affinity chromatography: suitable
immunoprecipitation (IP): suitable
capacity
~4 mg binding capacity (rabbit IgG)
shipped in
wet ice
storage temp.
−20°C
General description
EZview™ Red Protein A Affinity Gel is a highly visible, red colored Protein A affinity gel. The affinity resin contains Staphylococcus aureus Protein A covalently attached to crosslinked 4% agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue. The immune complexes, bound to the EZview™ Red Protein A Affinity Gel, are recovered by centrifugation. Red dye enhances visibility for more efficient results.
Application
EZview™ Red Protein A Affinity Gel has been used for purification of proteins in the preparation of immunoprecipitation and chromatin immunoprecipitation (ChIP) assays.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Features and Benefits
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Physical form
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
50% slurry in PBS with 50%Glycerol and 0.0015%
Storage and Stability
EZview™ Red Protein A Affinity Gel should be stored in 50% glycerol at -20 °C. This product is stable for at least one year when stored as indicated. Do not freeze in the absence of glycerol.
Legal Information
EZview is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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