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Key Documents

M5528

Sigma-Aldrich

Methyl Blue

certified by the Biological Stain Commission, powder

Synonym(s):

Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue

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About This Item

Empirical Formula (Hill Notation):
C37H27N3Na2O9S3
CAS Number:
Molecular Weight:
799.80
Colour Index Number:
42780
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Pricing and availability is not currently available.

Product Name

Methyl Blue, certified by the Biological Stain Commission

grade

certified by the Biological Stain Commission

Quality Level

form

powder

technique(s)

microbe id | staining: suitable

solubility

H2O: 1 mg/mL, blue

εmax

≥700 at 594-610 nm in 5 mM HCl

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2

InChI key

MCPLVIGCWWTHFH-UHFFFAOYSA-L

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Suitability

Certified as aniline blue for use in Mallory′s connective tissue stain and as a substitute for fast green FCF in Gomori′s one-step trichrome procedure.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

>572.0 °F - Pensky-Martens closed cup

Flash Point(C)

> 300 °C - Pensky-Martens closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Reactive oxygen species and cellular interactions between Mycosphaerella fijiensis and banana.
Jesus M de, et al.
Tropical Plant Biology, 4, 134-134 (2011)
Development of a bone reconstruction technique using a solid free-form fabrication (SFF)-based drug releasing scaffold and adipose-derived stem cells.
Lee JW, et al.
Journal of Biomedical Materials Research Part A, 101, 1865-1865 (2013)
Baogan Peng et al.
European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 16(1), 33-38 (2006-02-24)
This article was a preliminary report of prospective clinical trial of a group of patients with chronic discogenic low back pain who met the criteria for lumbar interbody fusion surgery but were treated instead with an intradiscal injection of methylene
Xiaoli Hou et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 66(3), 552-556 (2006-07-25)
A new synchronous fluorescence scan analysis was developed for the determination of HSA with high sensitivity with a triphenylmethane acid dye methyl blue as a fluorescence probe. When Deltalambda=140 nm, the synchronous fluorescence peak of methyl blue is located at
Sebastian Thaler et al.
Journal of cataract and refractive surgery, 37(6), 1147-1153 (2011-05-21)
To evaluate capsule-staining properties and biocompatibility of the triarylmethane dyes methyl blue and aniline blue compared with patent blue and trypan blue on cultured human corneal endothelial cells. Centre for Ophthalmology, University of Tuebingen, Tuebingen, Germany. Experimental study. Human corneal

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