This product has not been tested specifically for use in fungal staining in plant tissue. However, FITC-labeled wheat germ agglutinin or lectin has been cited in the literature for such applications. Please see the link below to review one such publication:
https://iris.unito.it/bitstream/2318/1740844/1/Carotenuto%20et%20al%202.pdf
L4895
Lectin from Triticum vulgaris (wheat)
FITC conjugate, lyophilized powder
Synonym(s):
WGA, Wheat germ agglutinin
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Pricing and availability is not currently available.
Recommended Products
conjugate
FITC conjugate
Quality Level
form
lyophilized powder
potency
<50 μg/mL agglutination activity
composition
Protein, ~50% Lowry
extent of labeling
~2 mol FITC per mol protein
storage temp.
2-8°C
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General description
Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.[1]
Application
For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine
Biochem/physiol Actions
Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.[1]
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Packaging
Package size based on protein content.
Physical form
Contains phosphate buffer salts
Preparation Note
Conjugates are prepared from affinity purified lectin.
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Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
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Please is this product use in staining Arbuscular mycorrhizal fungi in root, for confocal microscopy examination?
1 answer-
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Does Sigma provide a stain to visualize cell borders for human Cardiomyocytes derived from the GM23338 iPSC cell line?
1 answer-
There is no recommended stain for cells from this type of cell line. However, for visualizing typical cardiomyocyte cell borders, one may consider L4895, as referenced by Vanhoutte D et al, Nat Commun. 2021 Jun 24;12(1):3928. L4895 is Sigma-Aldrich Lectin from Triticum vulgaris (wheat). The article titled "Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy" in Nat Commun. 2021 Jun 24;12(1):3928 may serve as a point of reference.
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