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I1782

Sigma-Aldrich

Inosine Monophosphate Dehydrogenase Type II human

recombinant, expressed in E. coli

Synonym(s):

IMP:NAD oxidoreductase, IMPDH II

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About This Item

Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

form

solution

specific activity

≥0.05 units/mg protein

mol wt

~55 kDa

packaging

vial of ≥0.002 unit

UniProt accession no.

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... IMPDH2(3615)

General description

Inosine Monophosphate Dehydrogenase Type II (IMPDH2) is a ubiquitously expressed dominant isoform during developmental stages. IMPDH2 gene is mapped to human chromosome 3p21.31.

Application

Inosine Monophosphate Dehydrogenase Type II human has been used to test the inhibitory effect on vacor adenine dinucleotide (VAD) on its dehydrogenase activity.

Biochem/physiol Actions

Inosine Monophosphate Dehydrogenase Type II (IMPDH2) binds to adenosine triphosphate (ATP) and guanosine triphosphate (GTP). It catalyzes the formation of xanthosine monophosphate from inosine monophosphate in the presence of nicotinamide adenine dinucleotide (NAD). IMPDH2 elevated levels in tumors are correlated to its rate-limiting activity in guanosine monophosphate (GMP) synthesis. High levels of IMPDH2 is implicated in glioblastoma (GBM). It is regarded as a potential therapeutic target against tumors, antiviral, and immunosuppression-related pathologies.
Type II is the predominant IMPDH isoform and is specifically linked to a wide range of cancers and lymphocyte proliferation.

Unit Definition

One unit will produce 1.0 μ mole of XMP from IMP with corresponding reduction of β-NAD per minute at pH 8.0 at 25 °C.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, containing 0.5 mM EDTA and 1 mM DTT.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rebecca R Midtkandal et al.
Bioorganic & medicinal chemistry letters, 22(16), 5204-5207 (2012-07-17)
2-Deoxy-C-nucleosides are a subcategory of C-nucleosides that has not been explored extensively, largely because the synthesis is less facile. Flexible synthetic procedures giving access to 2-deoxy-C-nucleosides are therefore of interest. To exemplify the versatility and highlight the limitations of a
Bertrand Daignan-Fornier et al.
Cells, 8(1) (2019-01-20)
Purine nucleotides are involved in a multitude of cellular processes, and the dysfunction of purine metabolism has drastic physiological and pathological consequences. Accordingly, several genetic disorders associated with defective purine metabolism have been reported. The etiology of these diseases is
Travis J Loya et al.
Nucleic acids research, 40(15), 7476-7491 (2012-05-09)
The yeast IMD2 gene encodes an enzyme involved in GTP synthesis. Its expression is controlled by guanine nucleotides through a set of alternate start sites and an intervening transcriptional terminator. In the off state, transcription results in a short non-coding
Lizbeth Hedstrom
Critical reviews in biochemistry and molecular biology, 47(3), 250-263 (2012-02-16)
The inosine monophosphate dehydrogenase (IMPDH)/guanosine monophosphate reductase (GMPR) family of (β/α)(8) enzymes presents an excellent opportunity to investigate how subtle changes in enzyme structure change reaction specificity. IMPDH and GMPR bind the same ligands with similar affinities and share a
Craig D Kaplan et al.
PLoS genetics, 8(4), e1002627-e1002627 (2012-04-19)
Structural and biochemical studies have revealed the importance of a conserved, mobile domain of RNA Polymerase II (Pol II), the Trigger Loop (TL), in substrate selection and catalysis. The relative contributions of different residues within the TL to Pol II

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