45.6.TG1.7

90110506, mouse blood, Lymphoblast

• UNSPSC Code: 41106514

Properties

• Product Name: 45.6.TG1.7, 90110506, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• biological source: mouse blood
• packaging: tube of 5 μg 90110506-DNA-5UG; pkg of vial of cells 90110506-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast
• products: Immunoglobulin G (IgG2b) ( kappa)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse BALB/c myeloma

Cell Line Description

Derived from a female BALB/c myeloma tumour MPC-11. The parental cell line has mutated during growth in 6-thioguanine to establish the drug resistant line 45.6.TG1.7. This line is HPRT negative and produces mouse IgG2b kappa immunoglobulins.

Application

Fusion partner, immunoglobulin production

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 4-5x100,000 cells/ml; 5% CO₂; 37°C. Cells achieve a maximum cell density of 1-1.5x1,000,000 cells/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.