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C8274

Sigma-Aldrich

Cytohelicase from Helix pomatia

lyophilized powder

Synonym(s):

Snail gut enzyme

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

composition

Protein, 50-80% Lowry

storage temp.

2-8°C

Application

Cytohelicase from Helix pomatia has been used:
  • in the enzyme solution for preparing the cell nuclei for immuno fluorescent in situ hybridization (immunoFISH)
  • in the enzyme mix to analyze meiotic cells at diakinesis and later stages in anthers
  • in the enzyme mix for the analysis of mitosis in anthers

Cytohelicase from Helix pomatia has been used to study the isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of Helix pomatia.
The enzyme from Sigma has been used for cytohelicase assay. The enzyme assay was used while studying the effects of MNN10 gene-disruption on protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae cultures.

Biochem/physiol Actions

Cytohelicase from Helix pomatia is used in the preparation of synaptonemal complex spreading techniques along with bovine serum albumin. It plays an important role in the digestion of cell wall. Cytohelicase interacts with chromatin and improves the adhesion of synaptonemal complexes to the hydrophobic plastic film.

Analysis Note

Contains glucanase/laminarinase activity

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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ImmunoFISH: simultaneous visualisation of proteins and DNA sequences gives insight into meiotic processes in nuclei of grasses.
Sepsi A, et al.
Frontiers in Plant Science, 9(7), 1193-1193 (2018)
The effect of bovine serum albumin and cytohelicase on surface-spread synaptonemal complexes of Rye (Secale cereale).
De Jong JH and van Eden J
Stain Technology, 63(4), 213-220 (1988)
Improved method of enzyme digestion for root tip cytology.
Lattier JD, et al.
Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 52(7), 1029-1032 (2017)
Simon Amiard et al.
PloS one, 9(1), e86220-e86220 (2014-01-28)
In striking contrast to animals, plants are able to develop and reproduce in the presence of significant levels of genome damage. This is seen clearly in both the viability of plants carrying knockouts for key recombination and DNA repair genes
Sònia Garcia et al.
BMC plant biology, 12, 95-95 (2012-06-22)
In plants, the 5 S rRNA genes usually occur as separate tandems (S-type arrangement) or, less commonly, linked to 35 S rDNA units (L-type). The activity of linked genes remains unknown so far. We studied the homogeneity and expression of

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