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A4540

Sigma-Aldrich

Anti-Mouse IgM (μ-chain specific)−Agarose antibody produced in goat

IgG fraction of antiserum, saline suspension

Synonym(s):

Anti-Mouse IgM, Goat Anti-Mouse IgM

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

agarose conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

saline suspension

species reactivity

mouse

extent of labeling

≥5 mg per mL

technique(s)

Ouchterlony double diffusion: suitable

capacity

0.4 mg binding capacity

storage temp.

2-8°C

target post-translational modification

unmodified

General description

IgM exists as a pentamer and is secreted by peritoneal B cells. IgM is a highly conserved antibody in vertebrates and is expressed early during immune response.
IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory. This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice.
Goat anti-mouse IgM (μ-chain specific)-agarose antibody is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses), and IgM myeloma proteins.

Application

Anti-Mouse IgM (μ-chain specific)−Agarose antibody produced in goat has been used in agarose binding assay in primary splenic cells and in immunoprecipitation of soluble lens protein and myelogenous leukemia cell lines.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Goat anti-mouse IgM (μ-chain specific)-agarose antibody has been used for immunoprecipitation and ChIP assays. The product can also be used for Ouchterlony double diffusion applications.
To isolate chimeric proteins that were fused with the IgM heavy chain, CHO supernatant was ran over a column packed with agarose-linked goat anti-mouse IgM mu chain specific.

Biochem/physiol Actions

IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules. IgM has sites for N-linked glycosylation, with sugars namely, mannose, galactose, N-acetyl glucosamine and sialic acid IgM plays a key role in the engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM linked agarose resin have been tested for efficient conjugate binding.

Physical form

Suspension in 0.5 M NaCl containing preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A role for lengsin, a recruited enzyme, in terminal differentiation in the vertebrate lens
Wyatt K, et al.
The Journal of Biological Chemistry, 283(10), 6607-6615 (2008)
James P Hewitson et al.
Journal of immunology (Baltimore, Md. : 1950), 187(9), 4764-4777 (2011-10-04)
Heligmosomoides polygyrus is a widely used gastrointestinal helminth model of long-term chronic infection in mice, which has not been well-characterized at the antigenic level. We now identify the major targets of the murine primary Ab response as a subset of
O-GlcNAc transferase and O-GlcNAcase interact with Mi2beta at the Agamma-globin promoter
Zhang Z, et al.
The Journal of Biological Chemistry, jbc-M116 (2016)
David L Pflugh et al.
Journal of immunology (Baltimore, Md. : 1950), 169(9), 5130-5136 (2002-10-23)
Most hemopoietic cells express one or more members of the Ly-6 supergene family of small glycosylphosphatidylinositol-linked proteins. Although levels of Ly-6 proteins vary with stages of differentiation and activation, their function largely remains unknown. To ascertain whether ligands for Ly-6
Yingjie Zhang et al.
Journal of cellular biochemistry, 102(5), 1256-1270 (2007-05-02)
CCAAT/enhancer binding proteindelta (C/EBPdelta) gene transcription is highly induced in G(0) growth arrested mammary epithelial cells and "loss of function" alterations in C/EBPdelta have been reported in human breast cancer. To gain a better understanding of the positive and negative

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