![N-[Nα,Nα-Bis(carboxymethyl)-L-lysine]-12-mercaptododecanamide ≥90.0% (TLC)](/deepweb/assets/sigmaaldrich/product/structures/421/384/d2cb2cfc-59fa-44f9-832b-65e9af62ebaf/640/d2cb2cfc-59fa-44f9-832b-65e9af62ebaf.png)
There should be no difficulty with the use of the product to label a HIS-tagged recombinant protein as long as the HIS-6 tag is in an acceptable position for binding. Typically, this is done with either a N or a C-terminal His6 tag. Please note that the only tests that we conduct on this material include a solubility, purity and a luminescence assay. Hence, we do not offer a detailed protocol for use of the product for HIS-tag protein labeling.
39625
NTA - Atto 488
BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE)
Synonym(s):
Atto 488 - Nitrilotriacetic acid, complexed to Ni2+ - ion, Nα,Nα-bis(carboxymethyl)-L-lysine, Nickel(II) complex
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About This Item
UNSPSC Code:
12171501
NACRES:
NA.32
Pricing and availability is not currently available.
Recommended Products
product line
BioReagent
Quality Level
Assay
≥80% (sum of isomers, HPCE)
form
solid
fluorescence
λex 501 nm; λem 523 nm in PBS with 0.08% sodium azide
suitability
suitable for fluorescence
detection method
fluorometric
storage temp.
−20°C
General description
find more information here
Application
for the fluorescence detection of HIS-tagged proteins
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1A - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 Inhalation
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Ni-NTA-Atto conjugates provide specific and highly sensitive detection of His-tagged fusion proteins.
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We have a his-tag labeled recombinant protein that we would like to have fluorescently labeled, before we use it to detect/label antibodies. I came across Ni-NTA-Atto Conjugates and I was wondering wether we could use this to label out his-tagprotein?
1 answer-
Helpful?
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What is the number of nickel ions present in product 39624? Is it mono-nta, tris-nta, or another form?
1 answer-
There is one nickel ion per molecule, but the exact structure is proprietary.
Helpful?
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What is the molecular weight of this product NTA-ATTO488?
1 answer-
The molecular weight of this product is 964 Da.
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