SCT275
BioTracker™ Green Late-Phase Autophagy Live Cell Probe
Human
Synonym(s):
autolysosome live cell probe, green autophagy probe
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.47
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product name
BioTracker™ Green Late-Phase Autophagy Live Cell Probe,
biological source
human
Quality Level
packaging
vial of 20 nmol
manufacturer/tradename
Millipore
technique(s)
cell culture | stem cell: suitable
flow cytometry: suitable
shipped in
2-8°C
storage temp.
2-8°C
General description
SCT275 is used to detect late phase autophagy in live cells. This small hydrophobic probe molecule is plasma membrane-permeable and incorporates in to the autophagosome. Following lysosome-autophagosome fusion, increased acidity in the autolysosome enhances fluorescence signal of the BioTracker™ Green Late-Phase Autophagy Live Cell Probe.SPECTRAL CHARACTERISTICS: Excitation λ: 405 nmEmission λ: 525 nmRecommended laser/filter:Ex 350 - 450 nmEm 500 nm - 560 nm
Features and Benefits
Fluorescence microscopyFlow cytometry
Target description
Autophagy is an intracellular degradation process, where dysfunctional proteins and organelles are degraded by a system of organelles. Aggregated dysfunctional proteins are surrounded by the double membrane to form an autophagosome. DALGreen, which is a small hydrophobic molecule, passes the plasma membrane of live cells and is incorporated in the autophagosome.
Storage and Stability
Store at -20 °C, protected from light.
Other Notes
SCT275 is a small hydrophobic molecule live cell probe that localizes to the autolysosome. The probe fluoresces green as the autolysosome environment acidifies, enabling the assessment of autophagy by fluorescence microscopy and flow cytometry.Add 20 uL DMSO to tube of SCT275 and pipet to dissolve to make a 1 mM stock solution. Dilute with culture medium to 0.1 - 1.0 uM to make working solution.
Legal Information
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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