MABT1357
Anti-IZUMO1 Antibody, clone Mab120
clone Mab120, from rat
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Mab120, monoclonal
species reactivity
mouse
packaging
antibody small pack of 25 μL
technique(s)
immunofluorescence: suitable
inhibition assay: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
mouse ... Izumo1(73456)
General description
Izumo sperm-egg fusion protein 1 (UniProt: Q9D9J7; also known as Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo) is encoded by the Izumo1 gene (Gene ID: 73456) in murine species. OBF is a single-pass type I membrane protein that is detected on sperm surface after the acrosome reaction. It is an essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. It can be present as a monomer, homodimer, or homooligomer. It is synthesized with a signal peptide (aa 1-21), which is cleaved off in the mature form, which has an extracellular domain (aa 22-319), a short transmembrane domain (aa 320-340), and a cytoplasmic domain (aa 341-397). The extracellular domain assumes a distinct boomerang shape when not bound to IZUMO1R/JUNO. Its interaction with IZUMO1R/JUNO triggers a conformation change, so that the IZUMO1 extracellular domain assumes an upright conformation. A region comprising of 13 amino acids (148-160) is important for its interaction with IZUMO1R. Mab120 is a fusion-inhibiting antibody that inhibits fertilization. It binds to a region designated as putative functional fragment (aa 5-113). (Ref.: Inoue, N et al. (2013). Development. 140(15); 3221-3229).
Specificity
Clone Mab120 is a rat monoclonal antibody that detects Izumo sperm-egg fusion protein 1 (IZUMO1) in murine species. It targets an epitope with in the N-terminal putative functional fragment.
Immunogen
Mouse spermatozoa.
Application
Anti-IZUMO1, clone Mab120, Cat. No. MABT1357, is a rat monoclonal antibody that detects Izumo sperm-egg fusion protein 1 and has been tested for use in Immunofluorescence, Inhibition assay, and Western Blotting.
Inhibition Analysis: A representative lot inhibited fertilization in an in vitro system. (Inoue, N., et. al. (2013). Development. 140(15):3221-9).
Western Blotting Analysis: A representative lot detected IZUMO1 in Western Blotting applications (Inoue, N., et. al. (2013). Development. 140(15):3221-9).
Immunofluorescence Analysis: A representative lot detected IZUMO1 in Immunofluorescence applications (Inoue, N., et. al. (2013). Development. 140(15):3221-9).
Western Blotting Analysis: A representative lot detected IZUMO1 in Western Blotting applications (Inoue, N., et. al. (2013). Development. 140(15):3221-9).
Immunofluorescence Analysis: A representative lot detected IZUMO1 in Immunofluorescence applications (Inoue, N., et. al. (2013). Development. 140(15):3221-9).
Research Category
Cell Structure
Cell Structure
Quality
Evaluated by Western Blotting in mouse testis tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected IZUMO1 in mouse testis tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected IZUMO1 in mouse testis tissue lysate.
Target description
~56 kDa observed; 44.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2a in PBS without azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Nicolas G Brukman et al.
eLife, 13 (2024-01-24)
The fusion of mammalian gametes requires the interaction between IZUMO1 on the sperm and JUNO on the oocyte. We have recently shown that ectopic expression of mouse IZUMO1 induces cell-cell fusion and that sperm can fuse to fibroblasts expressing JUNO.
Nicolas G Brukman et al.
The Journal of cell biology, 222(2) (2022-11-18)
Mammalian sperm-egg adhesion depends on the trans-interaction between the sperm-specific type I glycoprotein IZUMO1 and its oocyte-specific GPI-anchored receptor JUNO. However, the mechanisms and proteins (fusogens) that mediate the following step of gamete fusion remain unknown. Using live imaging and
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