Skip to Content
Merck
All Photos(1)

Documents

AB1033

Sigma-Aldrich

Anti-Versican Antibody, a.a. 1360-1439 of mouse versican

Chemicon®, from rabbit

Synonym(s):

GAG beta Domain

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ACAN(176)

General description

Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan., Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest.

Specificity

Mouse versican GAG beta domain.

Immunogen

Epitope: a.a. 1360-1439 of mouse versican
GST fusion protein containing amino acids 1360-1439 of mouse versican.

Application

Anti-Versican Antibody, a.a. 1360-1439 of mouse versican is an antibody against Versican for use in IP, WB, IH(P).
Immunohistochemistry (formally fixed, paraffin embedded): 10 μg/mL. Tissues must be treated with Chondroitinase before staining. {0.5U/mL in 100mM Tris-HCL pH 7.2-7.4, 30 minutes at room temperature} Then block and stain.

Western blotting: 0.5 μg/mL

Immunoprecipitation:

Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified
The immunoglobulin was affinity purified against the recombinant antigen and is presented in PBS. Anti-GST reactivity has not been adsorbed. Liquid in PBS.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeat freeze/thaw cycles.

Analysis Note

Control
Immunohistochemistry Positive control: surrounding cartilageWestern blotting positive control: total 13 day mouse embryo

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Early but not late pregnancy induces lifelong reductions in the proportion of mammary progesterone sensing cells and epithelial Wnt signaling.
Meier-Abt, F; Brinkhaus, H; Bentires-Alj, M
Breast Cancer Research null
Keiichi Asano et al.
Scientific reports, 7(1), 17225-17225 (2017-12-10)
The proteoglycan versican is implicated in growth and metastases of several cancers. Here we investigated a potential contribution of stromal versican to tumor growth and angiogenesis. We initially determined versican expression by several cancer cell lines. Among these, MDA-MB231 and
Natasha McRae et al.
International journal of molecular sciences, 18(12) (2017-12-07)
In Duchenne muscular dystrophy (DMD), a dysregulated extracellular matrix (ECM) directly exacerbates pathology. Glucocorticoids are beneficial therapeutics in DMD, and have pleiotropic effects on the composition and processing of ECM proteins in other biological contexts. The synthesis and remodelling of
Jie Hong Hu et al.
Arteriosclerosis, thrombosis, and vascular biology, 35(12), 2647-2656 (2015-10-24)
Prenatal deletion of the type II transforming growth factor-β (TGF-β) receptor (TBRII) prevents normal vascular morphogenesis and smooth muscle cell (SMC) differentiation, causing embryonic death. The role of TBRII in adult SMC is less well studied. Clarification of this role
Penny S Thomas et al.
PloS one, 7(4), e35539-e35539 (2012-04-27)
Bicuspid aortic valve (BAV) is the most common congenital cardiac anomaly in humans. Despite recent advances, the molecular basis of BAV development is poorly understood. Previously it has been shown that mutations in the Notch1 gene lead to BAV and

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service