C4 has an extinction coefficient of E1%/280 nM = 10.3. The buffer is PBS, pH 7.2.
204886
Complement C4, Human
Complement C4, Human, CAS 80295-48-3,is a native glycoprotein composed of three non-identical subunits of 93, 75, and 32 kDa linked by disulfide bonds.
Synonym(s):
Complement C4, Human
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Quality Level
Assay
≥90% (SDS-PAGE)
form
liquid
specific activity
>3X105 C4H50 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
dry ice
storage temp.
−70°C
General description
Native, human C4 complement component. Glycoprotein composed of three non-identical subunits of M.W. 93,000 (α), 75,000 (β), and 32,000 (γ) linked by disulfide bonds. Present in normal human serum at 400 µg/ml. On activation of complement via the classical pathway, the C1s subcomponent of the C1 complex is converted to an active serine protease that cleaves the C4 α-chain at peptide bond 77, resulting in the production of C4a (M.W. 8740) and C4b fragments (M.W. 193,000). The released C4a peptide is one of the three complement-derived anaphylatoxins. The nascent C4b fragment can form a covalent ester bond with target surfaces. This covalent attachment of C4b to target acceptors is required for continuation of activation via classical pathway.
Biochem/physiol Actions
≥60% of the C4 activity present in freshly drawn normal human serum on a mg to mg basis
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Other Notes
Janatova, J. 1983. Ann. N.Y. Acad. Sci. 421, 218.
Hammer, C.H., et al. 1981. J. Biol. Chem. 256, 3995.
Hammer, C.H., et al. 1981. J. Biol. Chem. 256, 3995.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Could you provide the spectrophotometric information for accurate concentration calculation using a Nanodrop after dialysis? I have previously been given the extinction coefficient of proteins at 1 mg/ml. Is this information available? Additionally, I am interested in knowing the original buffer in which this protein comes.
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