General description
DLAM-MεVproS-13CH3 Kit has 13CH3 isotopomer precursors and contains protocol instructions for creation of isotopically-labeled proteins.
Application
DLAM-MεVproS-13CH3 is an optimized kit to produce proteins with -13CH3 isotopomer on the methyl groups of methionine and valine residues. This kit has been tested with protein isotopic labeling in E. coli. It is used in TROSY NMR for revealing the mechanisms of dynamic molecular machines of N-terminal sequence
For detection of long-range nOes or for the NMR study of large proteins
Packaging
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
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Tomasz L Religa et al.
Science (New York, N.Y.), 328(5974), 98-102 (2010-04-03)
The proteasome catalyzes the majority of protein degradation in the cell and plays an integral role in cellular homeostasis. Control over proteolysis by the 20S core-particle (CP) proteasome is achieved by gated access of substrate; thus, an understanding of the
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that
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