808645
TSKgel® Chelate-5PW (10 µm) HPLC Columns
L x I.D. 7.5 cm × 7.5 mm, iminodiacetic acid phase
Synonym(s):
TSKgel® Chelate-5PW HPLC Column and Guardgel Kit
About This Item
Recommended Products
product name
TSKgel® Chelate-5PW HPLC Column, phase iminodiacetic acid, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size
material
stainless steel
product line
TSKgel®
manufacturer/tradename
Tosoh Chelate-5PW, 08645
extent of labeling
20 μmol/mL resin ligand density
parameter
≤20% organic solvent
≤3 M salt concentration
≤45 °C temperature
1.2 mL/min flow rate
10 bar max. pressure
technique(s)
HPLC: suitable
L × I.D.
7.5 cm × 7.5 mm
matrix
polymer particle platform
matrix active group
iminodiacetic acid phase
particle size
10 μm
pore size
1000 Å
operating pH
2-12
compatibility
technique used for affinity HPLC
separation technique
affinity
General description
TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.
Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.
Legal Information
guard column
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